Base-editing-mediated artificial evolution of OsALS1 in planta to develop novel herbicide-tolerant rice germplasms (Mol. Plant)
Plant Science Research WeeklyThe trait of herbicide tolerance allows farmers to use chemical means to eliminate weed competitors. Acetolactate synthase (ALS) is an enzyme targeted by more than 50 different herbicides. In order to generate novel herbicide tolerance traits, Kuang et al. used a base-editing artificial evolution approach,…
Chimeric activators and repressors define HY5 activity and reveal a light-regulated feedback mechanism (Plant Cell)
Plant Science Research WeeklyPlants take cues from the environment and decide when and how to regulate growth. When light is limiting, etiolated growth allows plants to reach the soil surface and gain access to light. After light perception, plants de-etiolate and go through a series of morphological and genetic changes. HY5 is…
Metabolic labeling of RNAs uncovers hidden features and dynamics of the Arabidopsis transcriptome (Plant Cell)
Blog, Plant Science Research WeeklyThe ability to directly sequence RNAs (RNA-seq) has revolutionized our understanding of gene expression, but it can miss or underestimate short-lived RNAs. Several methods have been developed to identify newly-synthesized mRNAs to provide a snapshot of transcription as it happens. Szabo present Neu-seq,…
Review: In vitro analytical approaches to study plant ligand-receptor interactions (Plant Physiol)
Plant Science Research WeeklyIt seems every other paper shows a nice diagram of a signaling cascade that includes a receptor interacting with its ligand. However, sometimes these diagrams are little more than speculation or guesswork. It’s not always easy to figure out if this interaction is real. Here, Sandoval and Santiago review…
Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy (eLIFE)
Plant Science Research WeeklyAdvances in microscopy have greatly informed our understanding of fundamental plant processes, but the germline cells in flowers have been hard to image as they are tiny and embedded within other tissues. Valuchova et al. present a method using light sheet fluorescence microscopy that allows live cell…
Review: Methods to visualize elements in plants (Plant Physiol)
Plant Science Research Weekly
Plant Science Research Weekly: February 14
February 14, 2020/in Blog, WWR Full Post /by Mary Williams
Review: Deep learning for plant genomics and crop improvement
One of the goals of plant science is to use the molecular phenotype (genome, transcriptome, proteome) to predict the…
Reciprocal cybrids reveal how organellar genomes affect plant phenotypes (Nature Plants)
Plant Science Research WeeklyA cybrid is a cytoplasmic hybrid; in other words, a cell or organism in which the cytosolic genomes (mitochondria and plastid) have a different origin than the nuclear genome; the nuclear genome is inherited solely from the paternal parent, and the cytosolic genomes from the maternal parent. Starting…
Accurate and versatile 3D segmentation of plant tissues at cellular resolution (bioRxiv)
Plant Science Research WeeklyAdvances in microscopy allow biologists to document and understand phenotypic changes at the tissue level. Despite this advancement, analysis of microscopic images to characterize changes in phenotypes is still under development. In this paper, Wolny et al. have developed an image analysis tool PlantSeg…
Expression atlas of Selaginella moellendorffii provides insights into the evolution of vasculature, secondary metabolism, and roots (Plant Cell)
Plant Science Research WeeklyLycophytes, including the model species Selaginella moellendorffii, are extant (still alive today) seedless vascular plants that were particularly abundant around 400-300 million years ago (and major contributors to the formation of coal). To further understand the biology of some of these oldest extant…