Tag: Research Methods and Tools

Auxin is an omnipresent, tryptophan-derived phytohormone that plays roles in innumerable physiological processes, so it is imperative to understand its distribution. Currently the best tools to do so employ the use of indirect sensors, such as the synthetic auxin-responsive promoter DR5. While invaluable,…

Gibberellin (GA) controls multiple developmental processes throughout the plant life cycle, from seed germination to flowering. In Arabidopsis, GA also regulates division of meristematic cells at the tip of the root and cell elongation in the growing zone. Despite its importance, little is known about…

The osmotic state of a cell is affected by internal (metabolite content) and external (water availability) factors. Recent work by Cuevas-Velazquez and colleagues describes the design of a FRET-based biosensor that allows dynamic monitoring of osmotic stress in living cells. They exploited features of…

Vilarrasa-Blasi and coworkers described their impressive work on a huge barcoded Chlamydomonas collection, in which they screened almost 60,000 insertion mutants grown under a wide range of environmental and chemical stress conditions. The authors assessed genotype-phenotype specificity for almost…

Gramene (www.gramene.org) is a curated, open-source, integrated data resource for comparative functional genomics in crops and model plant species. It launched 20 years ago and has continually grown and expanded to incorporate new tools and resources. This update by Tello-Ruiz et al. describes its…

Cis-regulatory elements (CREs) are DNA sequences found near or within genic regions that drive proper gene expression in time and space. Thus, CREs play essential roles in the diversification of spatially distinct cell-types with specialized function in multicellular organisms. To identify CREs underlying…

Producing a genetically-modified or -edited plant requires two distinct processes: DNA modification, followed by regeneration of a plant from the edited cells. The first process has been greatly enhanced by CRISPR/Cas9, but the second has continued to present challenges. Here, Debernardi et al. demonstrate…

CRISPR-Cas is a revolutionary technology that has taken the science of genetic manipulation to higher levels by leaps and bounds. In spite of the ease with which it is used for genome editing, the present delivery methods are not without shortcomings; undesirable effects on non-target regions and…