Recent Posts

TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity (Nature Comms)

Identification of protein- protein interactions helps understand the signaling cascade in multiple biological process including development, biotic and abiotic stress. Previously, a proximity-based labelling approach referred to as BioID had been used to identify proteins in close proximity to a protein…

Review: Methods to quantify primary plant cell wall mechanics ( J. Exp. Bot)

Plant growth and morphogenesis are linked to cell wall properties, so a deep understanding of cell wall biochemistry and mechanics is essential for studying plant development. In a recent review, Bidhendi and Geitmann describe current and emerging techniques for the analysis of cell wall mechanics. Classic…

Review: Imaging technologies to uncover the role of Ca2+ signaling in plant nutrient homeostasis (Plant Cell Environ)

Maintaining nutrient homeostasis is ridiculously challenging, in large part due to the tremendous effects individual nutrients have on other nutrients’ uptake and action, as well as the interactions between nutrient homeostasis and environmental conditions. In order to maintain the “right” amount…

How to be an effective mentor

Being an effective mentor involves making yourself available to support and advise someone when they need it, delivering that support in a way that makes sense to them, and always keeping that person's best interests in mind. In this webinar we discussed methods of mentorship and how to become a better…

Review: Crop breeding technologies to feed the world (Nature Biotech)

The world demands food, plant scientists and breeders have the challenge of feeding a growing population. In a recent review Hickey et al. summarize the state-of-the-art technologies used for crop improvement. In use since 2003, ‘speed breeding’ is a set of improved methods for fast-tracking plant…

Addressing fundamental questions in plant science through synthetic biology

About this seminar In this seminar, we examined the role that synthetic biology can play in answering fundamental questions in plant science. In studying plants for the past hundred years, we’ve learned a great deal about the mechanistic underpinnings of development and metabolism. However, the…

MapMan4: A refined protein classification and annotation framework applicable to multi-omics data analysis (Mol Plant)

Revolutions in omics technologies have rapidly increased the number of plant genomes, transcriptomes and proteomes available. However, protein sequences need to be assigned a biological function to provide useful insights for comparative genomics and transcriptomics. Here, Schwacke et al present MapMan4,…

Designer pentatricopeptide repeat PPR protein as RNA affinity tag (Plant Cell)

Pentatricopeptide repeat (PPR) proteins bind RNA, using a code in which specific amino acid motifs recognize specific bases, similar to TAL effector proteins. In plants, PPR proteins localize almost exclusively to mitochondria and chloroplasts, with functions including RNA, stabilization, splicing and…

Application of TurboID-mediated proximity labeling for mapping a GSK3 Kinase signaling network in Arabidopsis (bioRxiv)

In this preprint Kim et al., have provided an optimized method for determining protein-protein interaction in plants using TurboID-mediated labelling.  TurboID is an engineered promiscuous biotin ligase that marks proteins it comes in contact with. Identification of interacting partners of a protein…