In vivo gibberellin gradients visualized in rapidly elongating tissues
Blog, Plant Science Research Weekly, Research, Research BlogBiosensors can reveal cellular or even sub-cellular distributions of hormones, greatly enhancing our understanding of hormone action. Rizza et al. have developed an in vivo, FRET-based gibberellin biosensor, GIBBERELLIN PERCEPTION SENSOR 1 (GPS1), that incorporates portions of two GA-binding proteins,…
Imaging of Light Absorption and Photosynthesis
Blog, Plant Physiology: On The Inside, Research, Research BlogDue to its ease of use and noninvasive nature, variable chlorophyll fluorescence techniques have become increasingly popular for estimating photosynthetic parameters. Most measurements of variable chlorophyll fluorescence in complex plant tissues and surface-associated cell assemblages (e.g. biofilms)…
Nuclear transcriptomes at high resolution using retooled INTACT
Blog, Plant Science Research Weekly, Research, Research BlogReynoso et al. describe the Isolation of Nuclei from TAgged specific Cell Types (INTACT) in rice. In this method, modified from one described previously in Arabidopsis and tomato, nuclei are labeled with a biotinylated nuclear-envelope anchored protein. Best results were obtained when a rice WIP (WPP-Interacting…