Reynoso et al. describe the Isolation of Nuclei from TAgged specific Cell Types (INTACT) in rice. In this method, modified from one described previously in Arabidopsis and tomato, nuclei are labeled with a biotinylated nuclear-envelope anchored protein. Best results were obtained when a rice WIP (WPP-Interacting protein) was fused to GFP and BLRP (biotin ligase target peptide). The protein is biotinylated at the outer surface of the nucleus through a second transgene encoding biotin ligase. Biotin-labeled nuclei are then purified through the use of streptavidin-coated beads. The purified nuclei are sources of nuclear RNA and proteins for studies of gene regulation. Plant Physiol.
You might also like
Quantitative imaging to investigate regulators of membrane trafficking in Arabidopsis stomatal closure ($) Traffic
Ray parenchymal cells contribute to lignification of tracheids in developing xylem of Norway spruce (Plant Phys)
Expression atlas of Selaginella moellendorffii provides insights into the evolution of vasculature, secondary metabolism, and roots (Plant Cell)
Reprogramming of root cells during nitrogen-fixing symbiosis involves dynamic polysome association of coding and non-coding RNAs (Plant Cell)