Tag: Research Methods and Tools

Persistent drought monitoring using a microfluidic-printed electro-mechanical sensor of stomata in planta The study of stomatal dynamics is crucial for understanding photosynthetic gas exchange and plant hydraulics, but there are currently no methods for measuring the conductance of individual stomata…

Roots are essential plant organs that need to respond and adapt to dynamic environments. It is still unknown if these adaptations are based on systemic or cell-based responses.  Stanley and colleagues created the dual-flow-RootChip, a platform for the heterogeneous perfusion of Arabidopsis roots to…

Plant roots constantly engage in nutrient and water uptake for crop productivity. Increasing the nutrient uptake efficiency of roots will promote sustainable agriculture by decreasing the need for fertilizer applications. To achieve this task, we need to understand the physiology of intact roots in their…

If you ever tried to compare the results from two different experiments, you probably realized that small variations in the environment have big impact on how the plants grow. There is a lot to be learned from how the variation in the environment affects plant phenotypes, but currently the description…

Chlorophyll fluorescence is a common tool to investigate the behavior of the photosynthetic appratus, therefore photosynthetic capacity, at any physiological state. However, the different optical density of the samples can lead to light-dependent over- or underestimation of effective PSII quantum yields.…

New genome editing technology, such as zinc-finger nucleases and CRISPR/Cas9, are revolutionizing reverse genetics studies because they allow fast and precise genetic modifications in many species. However, they require efficient transformation and selection methods. This is notably a problem for algae…

Schmidt et al. demonstrate that nanopore technology can be applied to plant genomes https://doi.org/10.1105/tpc.17.00521 By Schmidt, M. H.-W., Vogel, A., Denton, A. K., Bolger, A. M., Bolger, M. E., and Usadel, B. Background: An organism’s genome contains all the necessary information for its…

Shendure et al. provide a superb review of how DNA sequencing technology has changed over the years and how these changes open up new applications. They start with the Maxam and Gilbert chemical cleavage and the Sanger “chain-termination” methods developed in the 1970s, and describe the scale-ups…