Entries by Vijaya Batthula

An inducible genome editing system for plants (Nature Plants)

To understand gene function, it is necessary to study loss-of-function phenotypes. However, knockouts of some genes are lethal, which makes it impossible to study their loss-of-function phenotype. Although here are inducible overexpression systems and inducible knockdown systems, as yet there has been no inducible knockout system. In this paper Wang et al. developed an inducible […]

Review: Feedback mechanisms between membrane lipid homeostasis and plant development (Dev. Cell)

Plant development is a regulated process of cell division, expansion, and differentiation. Membrane lipids are crucial to these processes, as illustrated in this review by Boutté and Jaillais. The authors discuss the major lipid components in the different membrane systems and how these vary in space and time during plant development. Taking examples from Arabidopsis […]

Reprogramming of stem cell activity to convert thorns into branches (Curr. Biol.)

Thorns are modified axillary shoots with a sharp tip that helps in deterring herbivores and are found among several families of angiosperms. Thorns develop from their meristem-like tip to their base, but unlike branches they are determinate organs. The mechanism of their terminal differentiation into a sharp structure is not known. In this paper, Zhang […]

Dual-sensing genetically encoded fluorescent indicators, ABA and second messenger dynamics (Plant Cell)

Responses to a changing environment require an interplay between primary and secondary messengers. Hormones like abscisic acid (ABA), auxin etc. act as a primary messengers, whereas molecules like Ca2+, reactive oxygen species (ROS), other cations and anions act as a secondary messenger. In this paper, Waadt et al. generated a set of dual-reporting genetically encoded […]

The biogenesis of CLEL peptides involves several processing events in consecutive compartments of the secretory pathway (eLIFE)

Small signaling peptides are cleaved from precursor proteins by the action of proteases and are also subject to other post-translational modifications. Subtilases (SBT) are mostly extracellular proteases, but SBT6.1 is membrane-localized at the Golgi and plasma membranes. Furthermore, it has been shown to cleave precursors of the CLE-Like (CLEL) peptides, specifically CLEL6 and 9, which […]

Post germination seedling establishment by ABA in response to light (Plant J.)

Abscisic acid (ABA), is implicated in reversible inhibition of seed germination (emergence of radicle) and post-germination seedling establishment (formation of green open cotyledons) during unfavorable conditions. Yadukrishnan et al. analyzed the role of light in ABA- mediated inhibition of post germination seedling growth and laid out a molecular framework using a combination of genetics and […]

How do auxin temporal dynamics regulate patterning? (eLIFE)

Auxin forms spatial gradients that are implicated in organ morphogenesis. However, it is not known how temporal auxin gradients are integrated with the spatial information. In this paper, Galvan-Ampudia, Cerutti et al., showed using a ratiometric quantitative auxin reporter (quantitative DII-VENUS) that auxin moves faster than cellular growth in the shoot apical meristem (SAM), and […]

How far does stomatal activator and inhibitor signaling work in the plant epidermis? (bioRxiv)

Stomata are the pores on the plant surface surrounded by a pair of guard cells that control gaseous exchange and water loss. Among the many genes involved in stomatal patterning and development, EPIDERMAL PATTERNING FACTOR 1 (EPF1) and STOMAGEN encode signaling peptides and acts as negative (inhibitor) and positive (activator) regulators of stomatal patterning respectively. […]