Review: DNA sequencing at 40: past, present and future ($)
Blog, Plant Science Research Weekly, Research, Research BlogShendure et al. provide a superb review of how DNA sequencing technology has changed over the years and how these changes open up new applications. They start with the Maxam and Gilbert chemical cleavage and the Sanger “chain-termination” methods developed in the 1970s, and describe the scale-ups…
De novo assembly of a new Solanum pennellii accession using nanopore sequencing
Blog, Plant Science Research Weekly, Research, Research BlogChromosomes are long, and DNA sequencing reads have typically been short, meaning that it is necessary to assemble lots and lots of short reads by looking for overlapping sequences. This strategy is made more difficult in repeat-rich and transposon-rich regions of genomes, which characterize many plant…
High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell
Blog, Plant Science Research Weekly, Research, Research BlogThe current version of the Arabidopsis thaliana Col-0 reference genome, TAIR10, still has some gaps and mis-assemblies due to centromeres and repeat-rich regions. In another demonstration of the promise of single-molecule sequencing, Michael, Jupe et al. used a single nanopore flow cell to sequence the…
Genome of wild olive and the evolution of oil biosynthesis
Blog, Plant Science Research Weekly, Research, Research BlogOlive oil is a staple of the healthy “Mediterranean diet” and contains high levels of the monounsaturated fat oleic acid. Unver, Wu et al. present the genome of the wild olive tree (Olea europaea var. sylvestris) (draft sequences of domesticated olive trees without extensive functional annotation…
The genome of Quenopodium quinoa, a halophytic pseudocereal
Blog, Plant Science Research Weekly, Research, Research BlogQuenopodiium quinoa is a highly nutritive and facultative halophyte pseudocereal whose cultivation has increased 10 fold in the last decades. However, the adaption to non-native areas is not easy to achieve and the limited genetic resources do not allow a breeding program. Zou and collaborators have…
Insights into land plant evolution garnered from the Marchantia polymorpha genome
Blog, Plant Science Research Weekly, Research, Research BlogThe liverwort Marchantia polymorpha is a fascinating plant for many reasons, including the fact that it is one of the earliest terrestrial species that split off from the rest of the land plants. Therefore, comparisons between Marchantia and green algae or Marchantia and the rest of the land plants tell…
Genomic and epigenomic events occurring during germination
Blog, Plant Science Research Weekly, Research, Research BlogSeeds integrate environmental information and trigger molecular pathways that help with the decision making process of whether to germinate or not. Several studies in numerous species have shown details of global transcriptomic changes during germination. However, Narsai et al. dig a little deeper. By…
Completing the whole puzzle of whole genome duplications in land plants
Blog, Plant Science Research Weekly, Research, Research BlogA hot topic in plant evolutionary biology is whole genome duplications (WGDs), in which an organism copies its entire genetic dataset. Having double the required DNA is often viewed as detrimental but can be useful in times of rapid environmental change. Recently, the role of WGDs during plant evolution…
An acidophilic green algal genome provides insights into adaptation to an acidic environment ($)
Blog, Plant Science Research Weekly, Research, Research BlogHirooka et al. examined the genome of an acid-loving green alga, Chlamydomonas eustigma, to learn how it tolerates its low pH environment. Key differences between the acidophilic species and the neutrophilic species Chlamydomonas reinhardtii include: an increase in expression of genes encoding plasma…