Live Confocal Imaging of Developing Arabidopsis Flowers

Monitoring cells and their activities in real time provides exceptional insights into their mechanisms and strategies of growth. Prunet has used live confocal imaging of Arabidopsis flowers to study the placement of floral organs and the dynamics of floral stem cells. Here, he presents a detailed protocol for live confocal imaging of developing Arabidopsis flowers. The included video will help new imagers learn the fine points of this powerful method. JOVE 10.3791/55156