Tag: Development

The shoot apical meristem (SAM) maintains a small pool of pluripotent stem cells, which are the source of all above-ground plant tissues. In Arabidopsis thaliana, CLAVATA3 (CLV3), CLAVATA1(CLV1) and RECEPTOR-LIKE PROTEIN KINASE2 (RPK2) repress WUSCHEL (WUS) expression. WUS induces CLV3, forming a local…

The vascular plant body composed of root and shoot is specified during embryogenesis, but most flowering plants can also develop additional root systems post-embryonically: lateral roots, as a response to wounding, or shoot-borne roots (“adventitious” roots, literally meaning “in the wrong place”).…

Live Plant Cell Tracking: A Fiji plugin to analyze cell proliferation dynamics and understand morphogenesis Morphogenesis in plants occurs in 3D space over time, referred to as 4D. Recent advances in high-resolution microscopy imaging and computational pipelines have helped to better understand…

Single-cell RNA sequencing (scRNA-seq) is a powerful tool to identify rare and novel cell types, characterize multiple cell types, and more accurately understand their functions across their life cycles. The Arabidopsis root is a perfect system in which to investigate cellular differentiation due to…

Angiosperm seed formation is an incredibly complex process, considering that it requires coordinating the development of three distinct structures –the embryo, the endosperm and the seed coat– in a small space. However, Doll and Ingram show us that angiosperms faced this challenge by evolving a complex…

Water loss during photosynthesis is limited by specialized plant cells such as stomata, as well as cuticle formation on the epidermis. In Arabidopsis thaliana, stomata lineage is a good system in which to elucidate the interplay between biochemical signals and mechanical properties during leaf development.…

Leaf features have undergone significant changes during the course of evolution and domestication owing to ecological adaptations and changes in source-sink dynamics. Therefore, wild relatives of crop plants are important resources for exploring the genetic regulation of leaf features that might not…

In this seminal paper, Roszak et al. present a deeper understanding of the development of the protophloem cell lineage. The differentiation process from the phloem stem cell to the enucleated cell state spans approximately 19 cells. Using live imaging, FACS sorting and single-cell RNA sequencing, the…