Control of gene expression involves both activation and repression of transcription. Schaumberg et al. used transient expression in Arabidopsis and sorghum protoplasts and dual-luciferase outputs to rapidly quantitate synthetic repressors and repressible promoters, and verified their results in transgenic Arabidopsis plants. Nature Methods 10.1038/nmeth.3659
Author Archive for: Mary Williams
About Mary Williams
FEATURES EDITOR, THE PLANT CELL
I studied Biochemistry at Berkeley (BA), Plant Molecular Biology at Rockefeller (PhD), did a postdoc at Berkeley with Ian Sussex, and then spent 14 years as a Biology professor at Harvey Mudd College in Claremont, California. In 2009 I started working at the American Society of Plant Biologists as Features Editor of the journal Plant Cell and the developer of Teaching Tools in Plant Biology. My passion lies in making it a little bit easier for students of all ages to understand plants and plant science research. I live in Glasgow, Scotland, with my family and our much adored dog.
Entries by Mary Williams
Patron et al. describe the need to standardize DNA parts and terminology, so that researchers and inventors can use off-the-shelf parts (they draw an analogy to the standardization of components in mechanical and electronic engineering). New Phytol. 10.1111/nph.13532
Liu and Stewart provide an overview of the design cycle for plant synthetic biology, driven by engineering principles. They describe components and design tools, and give examples of the application of synthetic biology including the design of biosensors and alterations of plant metabolic pathways. Trends Plant Sci. 10.1016/j.tplants.2015.02.004