Metabolites through the looking glass with CEST MRI
Plant Science Research WeeklyNon-invasive imaging technologies like computed tomography and magnetic resonance imaging (MRI) have revolutionized medicine by improving diagnostics and guiding treatment. Due to its versatility, MRI also holds potential for plant sciences, where it can be used to visualize and quantify metabolites…
An inducible protein degradation system for rapid depletion of target proteins
Plant Science Research WeeklyTargeting a given protein for degradation at a specific time can be very useful when investigating its function in the cellular context. In this paper, Huang et al. present an inducible protein degradation system tailored for plants, named E3-DART. The system is based on the specific interaction between…
Review: Integrating cellular electron microscopy with multimodal data to explore biology across space and time
Plant Science Research WeeklyFifty years ago (1974), Albert Claude, Christian de Duve, and George Palade were awarded the Nobel Prize for their discoveries on the structural and functional organization of the cell, which Claude eloquently framed by writing, “We have entered the cell, the mansion of our birth, and started the inventory…
Special issue: Human-machine collaboration in plant biology
Plant Science Research WeeklyThis is an excellent article to wrap up this year and lead us into the future. Introducing a special issue of Plant Cell Physiology, Nakajima et al. summarize an exciting collection of papers that look at diverse ways that plant biology can be enhanced through “human-machine collaborations”. Some…
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors
Plant Science Research WeeklyAlthough CRISPR/Cas9 tools have provided new opportunities for genome editing, using these systems to introduce large pieces of DNA has been challenging. A new genome editing technique, “PrimeRoot” (Prime editing-mediated Recombination Of Opportune Targets), was introduced by Sun et al. and shown…
Review: Proximity labeling in plants
Plant Science Research WeeklyGenetic studies can suggest that two proteins function in the same pathway, but how can we figure out if they share the same space? In this review, Xu et al. provide an overview of proximity labeling, a method to identify proteins that co-localize in space. Proximity labeling uses a biotin ligase which…
Gaining cis-elements contributed to enhanced expression of C4 genes
Plant Science Research WeeklyC4 photosynthesis is derived from C3 photosynthesis. When related genes are compared, those involved in C4 photosynthesis tend to be more highly expressed. To understand this phenomenon, Singh et al. undertook a very impressive approach to look at transcriptional regulation of essentially all the photosynthetic…
Seeing clearly: Plant anatomy through Katherine Esau’s microscopy lens
Plant Science Research WeeklyThis is a fun and interesting paper that combines two threads, a historical overview of the many contributions of Katherine Esau to plant anatomy (many of you have her book on your shelf) and the historical advancements in microscopy. Geitmann weaves these threads together by putting Esau’s drawings…
eINTACT – An effective system for isolating effector-recipient cells in plant tissues
Plant Science Research WeeklyBacteria can cause plant diseases by secreting small molecules called effectors. Studies of these effectors on host plants mostly have not accounted for the cellular complexity of plant tissues. The difficulty of detecting and isolating effector-recipient cells means that scientists mostly use bulk-infected…