Recent Posts

A chloroplast protein atlas reveals punctate structures and spatial organization of biosynthetic pathways

Chloroplasts are the location of key processes including photosynthesis, starch synthesis and lipid synthesis. However, many chloroplast proteins have unknown functions,  a problem that can in part by addressed through high-resolution localization data. Here, 1034 putative chloroplast- localized proteins…

Review: Proximity labeling in plants

Genetic studies can suggest that two proteins function in the same pathway, but how can we figure out if they share the same space? In this review, Xu et al. provide an overview of proximity labeling, a method to identify proteins that co-localize in space. Proximity labeling uses a biotin ligase which…

BIN2 signaling network constructed using proximity labeling and TurboID

The brassinosteroid (BR) signaling pathway is essential for plant growth and development. BRASSINOSTEROID-INSENSITIVE2 (BIN2) is a GSK3-like kinase, serving as a repressor of the BR signaling pathway. In the absence of BR, BIN2 phosphorylates BRASSINAZOLE-RESISTANT1 (BZR1) family transcription factors…

Protein-protein interaction landscape of transcription factors during gynoecium development in Arabidopsis (Mol. Plant)

The gynoecium is the female reproductive structure of flowering plants. This complex organ, composed of different tissues, is an excellent model for studying plant organ development. Although the gynoecium has been extensively studied from a genetic point of view and a few studies have been carried out…

A Contextualized Catalog: Proteomic analyses of plant CCVs

Dahhan et al. explore the clathrin-coated vesicle proteome in Arabidopsis By Dana A. Dahhan and Sebastian Y. Bednarek, Dept. of Biochemistry, UW-Madison Background: Trafficking of vacuolar and plasma membrane proteins and other macromolecules is essential for plant development, nutrient uptake,…

Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process (Plant Cell)

It’s like the plot of every spy movie you’ve ever seen. If you could infiltrate your opponent’s headquarters what would you target to disable your enemy? This is the question addressed in new work by Petre et al. More than ten years ago, a family of small proteins was identified, the RXLR proteins…

Multi-omics analysis reveals interplay between BR and TORC signaling (bioRxiv)

Plants have evolved well-coordinated crosstalk between different signaling pathways to respond and adapt to various environmental stresses. Brassinosteriods (BRs) and Target of Rapamycin Complex (TORC) have multiple roles, through transcription, translation and autophagy, to control the balance between…

Chromatin phosphoproteomics identifies an AT-hook motif protein involved in PAMP-triggered immunity (PNAS)

Mitogen-activated protein kinases (MAPKs) are conserved protein kinases in eukaryotes that participate in signaling from cytoplasmic to chromatin events to allow transcription reprogramming. MAPKs play prominent roles at the chromatin level. Rayapuram et al. report a chromatin-associated phosphoproteome…

Multidimensional gene regulatory landscape of a bacterial pathogen in plants (Nature Plants)

The outcome of a plant-bacteria interaction is determined by the bacterial virulence and plant immune systems. Individually, these systems, and to an extent their interactions, have been well studied. However, certain aspects of their interactions remain elusive, particularly how plant immunity affects…