Tag: Molecular Biology

Students learn that membrane proteins are inserted into the endoplasmic-reticulum (ER) membrane co-translationally, but this mechanism does not hold for so-called tail-anchored (TA) proteins which carry a single C-terminal membrane spanning domain and insert into the ER membrane post-translationally.…

Autophagy is a process of selective membrane trafficking that delivers cargo to the vacuole or plasma membrane for recycling or secretion. ATG8 is a small ubiquitin-like protein that is required for formation of the double-membrane enclosed autophagy vesicle, the autophagosome. The ATG8 gene family has…

The gene-editing technology CRISPR/Cas, which introduces double-strand breaks that are repaired by non-homologous end joining (NHEJ), is best known for the promise it holds in modifying an organism’s DNA without the introduction of exogenous genes. However, as Puchta describes (Curr. Opin. Plant Biol.…

IN BRIEF by Jennifer Lockhart [email protected] DNA is sculpted by several types of epigenetic modifications with profound effects on gene expression, development, and stress responses. Much less is known about the more than 100 chemical modifications shaping plant RNA, a topic explored in the newly…

Aluminum (Al) toxicity is an important agricultural problem, limiting crop production globally. Al toxicity causes a reduction in nutrient uptake, resulting in nutritional deficiency and leading to an overall reduction in shoot biomass and crop yield. Eekhout et al. discuss Al toxicity and strategies…

Plants perceive microbial pathogens though cell-surface receptors that recognize conserved microbial patterns such as flagellin. Previous studies have identified the WRKY family of transcription factors as contributors to Microbial-Associated Molecular Pattern (MAMP)-Triggered Immunity (MTI). Birkenbihl…

Ambient temperature can influence plant architecture, an effect termed thermomorphogenesis.  In A. thaliana, thermomorphogenesis phenotypes include stem elongation and changes in leaf elevation angles.  Increased auxin biosynthesis involving the transcription factor PIF4 is required for thermomorphogenesis,…

Although most flowering plants produce flowers with male and female parts, there are exceptions which are broadly classified as dioecious (two houses; each individual is male or female, usually associated with the presence of a sex chromosome) and monoecious (one house, separate male and female flowers…