MicroRNAs from the parasitic plant Cuscuta campestris target host messenger RNAs
Blog, Plant Science Research Weekly, Research, Research BlogThere’s only one thing worse than having a parasite suck the life juices out of you, and that’s having a parasite suck the life juices out of you while simultaneously injecting you with miRNAs to increase your vulnerability. Shahid et al. looked at microRNAs isolated from dodder (Cuscuta campestris),…
SPF45-related splicing factor for phytochrome signaling promotes photomorphogenesis by regulating pre-mRNA splicing in Arabidopsis ($)
Blog, Plant Science Research Weekly, Research, Research BlogLight is one of the environmental conditions which regulates plants’ development, but knowledge of how light-induced transcript accumulation occurs via transcriptional versus post-transcriptional mechanisms is limited. Xin et al. report Splicing Factor for Phytochrome Signaling (SFPS) as an interacting…
Establishment of photosynthesis is controlled by two distinct regulatory phases ($)
Blog, Plant Science Research Weekly, Research, Research BlogChloroplast biogenesis and leaf development are closely linked processes that have been difficult to tease apart, until recently. Dubreuil et al. used a pluripotent inducible cell line from Arabidopsis, basically plant stem cells, which develop chloroplasts on demand when exposed to light. Using this…
The histone H3 variant H3.3 regulates gene body DNA methylation in Arabidopsis thaliana
Blog, Plant Science Research Weekly, Research, Research BlogThe histone H3 variant H3.3 is distinguished by its expression throughout the cell cycle, while H3.1 is expressed predominantly during DNA replication. Genome-wide ChIP studies in plants have shown that H3.3 is associated with actively transcribed genes, and enriched near the transcriptional end sites.…
So Much Data, So Little Time: ePlant Steps into the Breach for Plant Researchers
Blog, Research, Research Blog, The Plant Cell, The Plant Cell: In Brief0 Comments
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The ever-increasing amount of data available to researchers has come with similarly increasing cognitive loads in efforts to use these data. Even when data sets are stored in well-curated databases, it can be time-consuming to master the specific tools harbored at each site and cumbersome to move between…
Cis and trans determinants of epigenetic silencing by Polycomb repressive complex 2 ($)
Blog, Plant Science Research Weekly, Research, Research BlogPolycomb repressive complex 2 (PRC2) stably regulates gene expression by initiating repressive histone methylation (specifically, trimethylation of histone H3 at Lys27, H3K27me3). Xiao et al. investigated how PRC2 targets the genes it silences. They identified several Polycomb response elements (PREs)…
Zygotic genome activation occurs shortly after fertilization in maize
Blog, Plant Science Research Weekly, Research, Research BlogA plant’s life begins with the fusion of haploid egg and sperm cells to produce a diploid zygote. Many of the processes that control early development are under the control of the maternal genome, but at some point there is a shift towards zygotic control. Chen et al. investigated when this shift occurs…
Distinct phases of Polycomb silencing to hold epigenetic memory of cold in Arabidopsis ($)
Blog, Plant Science Research Weekly, Research, Research BlogSome plants can only flower after a cold period. This cold period has been shown to cause epigenetic gene silencing of an inhibitor of flowering, which in Arabidopsis is encoded by FLC. Yang and Berry et al. explore the mechanism by which FLC is epigenetically silenced in response to cold. Previous studies…
CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping ($)
Blog, Plant Science Research Weekly, Research, Research BlogProtein-protein interactions are crucial to our understanding of biology but can be hard to detect. Trigg et al. developed a sophisticated yeast two-hybrid assay augmented with Cre recombinase (CrY2H-seq) to identify the Arabidopsis transcription factor protein-protein interactome. In this method,…