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A pair of papers that redefines the pyrenoid, the eukaryotic CO2-concentrating organelle
Blog, Plant Science Research Weekly, Research, Research BlogPhotosynthesis in aquatic organisms is made difficult due to the low solubility of CO2 in water. Algae such as Chlamydomonas rheinhardtii overcome this limitation through a carbon-concentrating organelle called a pyrenoid. Two papers in Cell redefine our understanding of the pyrenoid structure. Mackinder…
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SIEVE ELEMENT-LINING CHAPERONE 1 restricts aphid feeding on Arabidopsis during heat stress ($)
Blog, Plant Science Research Weekly, Research, Research BlogAphids are major pests that damage plants by sucking out phloem sap and as by acting as vectors in transmission of more than 300 different viruses. Kloth et al. used a high-throughput method involving video-tracking of aphid behavior on leaf discs to score 350 Arabidopsis accessions for aphid resistance.…
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Tracking effector delivery in Irish famine potato pathogen
Blog, Plant Science Research Weekly, Research, Research BlogPathogenic microbes interfere with the host cellular and physiological processes to promote infection. This interaction is monitored by pathogen molecules called effectors that either act in intercellular space or enter the host cells. Mechanisms underlying the uptake of these effectors are not fully…
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Special delivery: An independent secretion pathway for the delivery of cytoplasmic pathogen effectors
Blog, Plant Science Research Weekly, Research, Research BlogPathogenic microbes manipulate host plants by secreting effector molecules that interfere with immunity. Bacterial phytopathogens achieve this using specialized secretion apparatuses that act as molecular ‘hypodermic needles’ to inject effector proteins directly into plant cells. In comparison, effector…
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ABA-induced stomatal closure involves ALMT4, a phosphorylation-dependent vacuolar anion channel
Blog, Plant Science Research Weekly, Research, Research BlogChanges in guard cell turgor pressure result in stomatal opening and closing, balancing CO2 uptake with transpiration. These dynamics have been studied as early as the 1800s and much knowledge has been gained regarding the components involved in this process, yet we are still far from a unified model…
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Getting It Done On Time: How Maize Orders DNA Replication
Research, The Plant Cell, The Plant Cell: In a NutshellWear et al. examine replication programs in plants. The Plant Cell 2017. https://doi.org/10.1105/tpc.17.00037
By William Thompson, Emily Wear, and Linda Hanley-Bowdoin
DNA replication is fundamental to all life, as it is the process by which genetic material is duplicated so it can be passed from…
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Review: Mechanosensitive ion channels ($)
Blog, Plant Science Research Weekly, Research, Research BlogIon channels are proteins that regulate the passage of ions across membranes. Ion channels are regulated in diverse ways – some are calcium-regulated, some are pH sensitive, some are charge sensitive, and some are sensitive to mechanical stimulation i.e., membrane tension. Basu and Haswell review mechanosensitive…
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Three-dimensional analysis of chloroplast structures associated with virus infection
Blog, Plant Science Research Weekly, Research, Research BlogIt’s not uncommon for viruses to manipulate the host’s membrane system to create protected structures for viral replication. These viral replication complexes (VRCs) “are thought to shield viruses from host defense systems such as RNA silencing, and … provide a microenvironment for enriching…
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Detection of nucleic acid–protein interactions in leaves using fluorescence lifetime imaging microscopy ($)
Blog, Plant Science Research Weekly, Research, Research BlogFRET-FLIM (Fluorescence Resonance Energy Transfer – Fluorescent Lifetime Imaging Microscopy) is a method that allows detection of interactions between two fluorescently-tagged molecules. Camborde and Jauneau et al. describe the use of FRET-FLIM to identify interactions in leaves between GFP-tagged…