Research

Plants use light as a source of energy and information; however, they are also sensitive and respond to  light/dark diurnal cycling, with many processes happening during the dark phase of the diurnal cycle. In this review, Seluzicki et al. emphasize the importance of studying and understanding what…

The interaction between angiosperms and their pollinators provides an excellent system to study co-evolution, and underpins the evolution of the biosynthesis of numerous interesting and useful specialized metabolites, from pigments to fragrances. Borghi et al. review the metabolic pathways that produce…

IN BRIEF: Nancy R. Hofmann [email protected] Plant development and responses to the environment hinge on the ability to target proteins to different areas of the plasma membrane within a cell. Indeed, the establishment of polar distributions of proteins such as PIN auxin transporters is among the…

Magnaporthe oryzae is the fungus that causes rice blast disease, which is a serious threat to food security. Ortiz et al. explore the interaction between AVR-Pia, a fungal effector protein, and RGA5, a rice NLR (nucleotide-binding domain and leucine-rich repeat protein) immune receptor protein. RGA5…

A seed undergoes a dramatic transformation from dried tissues into a mature plant.  This change requires energy produced by oxidative metabolism, but dry seeds contain dormant promitochondria that need to be reactivated.  Paszkiewicz et al. use bioimaging tools for an in vivo analysis of seed mitochondria…

Several studies have implicated microRNAs in plant defenses against pathogens. Wu et al. previously showed that virus resistance is facilitated by the interaction of viral-inducible AGO18 (which is cleavage-inactive) and rice miR528. In this work, they identified the role of miR528 in viral resistance.…

Students learn that membrane proteins are inserted into the endoplasmic-reticulum (ER) membrane co-translationally, but this mechanism does not hold for so-called tail-anchored (TA) proteins which carry a single C-terminal membrane spanning domain and insert into the ER membrane post-translationally.…

Nakamura et al. have created a searchable database, CATchUP (http://plantomics.mind.meiji.ac.jp/CATchUP) that allows the user to explore the spatiotemporal expression of genes across eight plant species (monocots and dicots) using data from publically available databases of large-scale RNA-Seq data.…

Efficient photosynthesis demands that plants have the capacity to capture photons when they are scarce, but at the same time not suffer damage from capturing more light energy than they are able to assimilate. To accomplish this, leaves need to be able to differentiate between a steady light level and…

Root architecture and anatomy contribute to water uptake efficiency and plant performance under water-limitation. Prince et al. explored root anatomy in a panel of soybean, and identified metaxylem number as a key trait influencing performance under water-limiting conditions. Increases in metaxylem number…

Understanding plant responses to water deficit is crucial for the development of drought-reliance, but complicated by the different ways plant researchers induce water deficit. Starting with the premise that transcription factors are important coordinators of water-deficit responses, Seeve et al. carried…

Autophagy is a process of selective membrane trafficking that delivers cargo to the vacuole or plasma membrane for recycling or secretion. ATG8 is a small ubiquitin-like protein that is required for formation of the double-membrane enclosed autophagy vesicle, the autophagosome. The ATG8 gene family has…

Our way of life depends on the chemical manufacture of thousands of products. Some of these can be produced through biomanufacturing, which may involve starting with a biological starting material, or using an enzyme or organism as catalyst. Advances in synthetic biology, metabolic engineering and genomics…

Iron (Fe) is a frequently limiting nutrient for plant growth. Iron uptake requires that plants manipulate the extracellular environment through secretion of protons, chelators and other molecules. Curie and Mari review new studies on the importance of coumarin (phenolic compound) secretion in iron uptake,…

Saline soils are an ever growing problem, whether due to seawater incursions, for example in Bangladesh, or due to the rising water table in Australia. Salinity is harmful in three ways: non-specific osmotic effects, excess Na+, and excess Cl–. Of these, chloride has received the least attention. Li…

The transpiration rate of water though stomata, known as stomatal conductance or gs is one of the most critical and regulated of plant physiological processes.  Buckley reviews recent progress on the development of comprehensive models of stomatal conductance, including the effect of soil moisture and…

The gene-editing technology CRISPR/Cas, which introduces double-strand breaks that are repaired by non-homologous end joining (NHEJ), is best known for the promise it holds in modifying an organism’s DNA without the introduction of exogenous genes. However, as Puchta describes (Curr. Opin. Plant Biol.…

IN BRIEF by Kathleen L. Farquharson [email protected] Limiting shoot growth is an important survival strategy for plants during times of drought; smaller leaves mean that less water is lost through transpiration and more is retained in the soil. As drought stress restricts both cell division and…

IN BRIEF by Jennifer Lockhart [email protected] DNA is sculpted by several types of epigenetic modifications with profound effects on gene expression, development, and stress responses. Much less is known about the more than 100 chemical modifications shaping plant RNA, a topic explored in the newly…

Phosphorus (P) is an important macronutrient for crop productivity.  In cereal crops like rice, about 60-85% of total plant P is allocated to grains and therefore removed from fields at harvest. Furthermore, the major form of P in the grains is phytate (C6H18O24P6), which cannot be digested by humans…

Aluminum (Al) toxicity is an important agricultural problem, limiting crop production globally. Al toxicity causes a reduction in nutrient uptake, resulting in nutritional deficiency and leading to an overall reduction in shoot biomass and crop yield. Eekhout et al. discuss Al toxicity and strategies…

The Solanaceae family is, nowadays, one of the most valuable for humankind as it contains several economically important crops as potato, tobacco, tomatoes, etc. However, fossil records that help as understand how and where this family has evolved are elusive. In this paper, Wilf et al. walk us through…

Much of the data used to predict forest responses to climate change comes from unfragmented forests, but much of the world’s forests are highly fragmented. Reinmann and Hutyra examined edge effects in a temperate forest in New England, and observed both an increase in biomass with proximity to the…

The Amazonian forest is an enormous and crucial ecosystem that encompasses a huge proportion of Earth’s biodiversity and stored carbon. By overlaying maps showing forest inventory plots and sites of ancient human impact, McMichael et al. observe that the inventoried plots from which we draw conclusions…

The Amazon rainforest stores 30% of land-based ecosystem carbon. How are carbon stores affected by selective tree removal and subsequent regrowth? Stored carbon continues to be lost for several years after logging due to damage-associated mortality of surviving trees. Piponoit et al. use data from more…

Disease-causing organisms attack as a population of diverse individuals. Is it possible to reduce the virulence of this population by introducing less-virulent individuals?  Some studies have shown such an effect; as an example, application of low-virulence strains of Aspergillus flavus can protect…

Two papers in Current Biology examine the genetics behind plant-pollinator interactions , focusing on genes controlling floral scent. Amrad et al. 10.1016/j.cub.2016.10.023 explore differences between bee, hawkmoth and hummingbird pollinated species of Petunia. They identify changes in expression in…

The autophagosome, a cellular compartment involved in the turnover of macromolecules, contributes to nutrient homeostasis, stress resilience and defense. Although several proteins have been identified as contributing to autophagosome formation and function, the precise origins of the autophagosome have…

Plants perceive microbial pathogens though cell-surface receptors that recognize conserved microbial patterns such as flagellin. Previous studies have identified the WRKY family of transcription factors as contributors to Microbial-Associated Molecular Pattern (MAMP)-Triggered Immunity (MTI). Birkenbihl…

When a plant species is introduced to a new region, it brings with it “hitchhikers” – other associated organisms. Sometimes, these hitchhikers negatively impact the environment into which they are introduced, for example by facilitating the host’s invasiveness, or through direct detrimental effects…

Orphan legumes, which include cowpea (Vigna unguiculata), Bambara groundnut (V. subterranea), grass pea (Lathyrus sativus) and marama bean (Tylosema esculentum), are important food sources for many farmers, but have largely been ignored by breeders and industry. Cullis and Kunert argue that some of these…

Coffee is a hugely popular beverage and contributes immeasurably to human productivity, but demand has risen by 50% in the past 20 years while coffee production is vulnerable to disease and climate change. Mehrabi and Lashermes observe that the popular arabica coffee comes from the plant Coffea arabica,…

Casadevall and Fang set out several proposals for research training to help ensure scientific “rigor”, which they define as promoting confidence in the truth or accuracy of the findings. The authors propose and elaborate on five foundations for scientific rigor: Redundancy in experimental design,…

Robust models of stomatal conductance are greatly needed to predict plant-atmosphere interactions in a changing climate, and to integrate new knowledge in physiology and ecological theory. Recent years have brought major advances in the experimental and theoretical understanding underpinning both process-based…

Due to plant cells’ shared walls and immobility, cell division, differentiation and expansion must be tightly regulated across space and time. The six members of the PLETHORA (PLT) transcription factor family distribute in gradients through the root tip and are largely responsible for its developmental…

Auxins are a class of phytohormones that regulate many aspects of plant development. Morales-Tapia and Cruz-Ramírez review the current status of the available auxin-driven computational models and discuss plausible integration of these into a single model for plant development. Computational modeling…

It has been argued that rising atmospheric CO2 levels might benefit plants by providing them more substrate for photosynthetic carbon-fixation. However, numerous studies have indicated that other factors interfere with a so-called CO2-fertilization benefit. Girardin et al. explore recent tree growth…

Heat stress is one of the main abiotic stresses plants encounter. Jiang et al. used combined transcriptomic and proteomic data to explore the responses of grape leaves to elevated temperatures (35, 40, 45°C). Using high-throughput sequencing and the iTRAQ (isobaric tags for relative and absolute quantitation)…

Ash dieback (a fungal disease) and the beetle Agrilus planipennis (a herbivore) are crushing ash tree populations in the Northern Hemisphere. To shed light on the genetic basis of the trees' susceptibility and to understand the genetic diversity of these trees, Sollars et al. have sequenced one individual…

The rice blast fungus Magnaporthe oryzae causes a devastating disease of rice that can reduce harvests by 30%. Infection of plant tissues by the fungus requires the formation of an appressorium, a specialized structure that builds up sufficient pressure to burst through the plant cuticle. Previous work…

Ambient temperature can influence plant architecture, an effect termed thermomorphogenesis.  In A. thaliana, thermomorphogenesis phenotypes include stem elongation and changes in leaf elevation angles.  Increased auxin biosynthesis involving the transcription factor PIF4 is required for thermomorphogenesis,…

Homeodomain transcription factors are well-known as regulators of developmental patterning, including in leaves. Andres et al. examine the molecular basis behind leaf shape in cotton, particularly the Okra locus that was identified by breeders as a regulator of leaf shape. They show that the Okra locus…

The AVP1 gene encodes a proton-pumping pyrophosphatase (H+-PPase) localized to the vacuolar membrane, which means that it pumps H+ into the vacuole using energy stored in pyrophosphatase (PPi). The direct consequences of its action are the acidification of the vacuole and the removal of PPi from the…

Legume nodules fix N, but their function has a high requirement for P, making nitrogen-fixation highly sensitive to P deficiency. Valentine et al. review how P limitation affects nodule function and also how nodules respond and adapt to P deficiency, drawing largely on studies of Virgilia divaricata,…

Nitrogen (N), phosphorus (P), and potassium (K) are the three macronutrients required in highest amounts for plant growth. N is abundant in the atmosphere, therefore plentiful if we overlook the energetic costs of converting N2 to usable form. By contrast, K and P are present in limited amounts in the…

Recently, we’ve been profiling first authors of Plant Cell papers that are selected for In Brief summaries. Here are the first-author profiles from the December issue of The Plant Cell. Yao-Pin Lin, featured author of Identification of Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis Current…

As a consequence of their ability to fix nitrogen in symbiosis with bacteria, legumes make key contributions to ecosystems and provide protein-rich foods for humans and other animals. Recent years have uncovered the nature of the signals involved in the cross-kingdom dialogues that occur between plant…

Tansy plants (Tanacetum vulgare L.) have diverse ethnobotanical uses including that of insect repellent, as a consequence of their production of volatile terpenes. Clancy et al. investigated the diversity of these emitted terpenes, and how they affect colonization by aphids and the ants that tend them…

It is widely stated that domestication has contributed to a decrease in plant resistance to herbivory, but to what extent is this true? In a contribution to a special issue on “Human influences on evolution”, Whitehead et al. describe results of their meta-analysis. Although their data show a consistent…

Cadmium (Cd) is among the most toxic heavy metal to humans. Contamination of Cd in soils poses a serious threat to both crop productivity and human health in many parts of the world. Understanding the molecular mechanisms of Cd transport process will help in developing plants for soil remediation and…

Soil saline-alkalization is a major abiotic stress to agriculture worldwide, causing considerable damage to crop growth and loss of crop productivity. In alkaline soil, iron availability to plants also becomes very limiting. This paper explores the physiological and molecular mechanisms of rice plant’s…

Although most flowering plants produce flowers with male and female parts, there are exceptions which are broadly classified as dioecious (two houses; each individual is male or female, usually associated with the presence of a sex chromosome) and monoecious (one house, separate male and female flowers…

Three PNAS papers explore DNA methylation and/or small RNAs in plant reproductive development. In angiosperms, sexual reproduction is accompanied by DNA demethylation in certain tissues, particularly those that have a nutritive or supporting role.  Park et al. (10.1073/pnas.1619047114) show that in…

Trehalose-6-phosphate (T6P) is a sugar signaling molecule that regulates how plants allocate and use sucrose, which in turn affects stress resilience and yields. Griffiths et al. designed a plant-permeable, photo-activated T6P analogue that is converted to T6P in planta. Spraying this compound onto plants…

In this entertaining Opinion article written for the 150th anniversary of Mendel’s seminal work, Bernardo reviews “bandwagons” that have come and gone in plant breeding. Early bandwagons such as induced polyploidy are discussed, as well as more recent trends such as trangenics, molecular markers…

The Journal of Experimental Botany has a special issue on vascular development that features several outstanding review and opinion articles. Topics include Evolution of Conducting Cells, Regulation of Vascular Cell Division, overviews of hormones, peptide signals, receptors and transcription factors…

Plants from the Solanaceae family had, and continue to have, fundamental roles in plant genetic research.  Gebhardt summarizes the historical contributions of the Solanaceous plants tomato, tobacco, petunia, potato, pepper, and eggplant.  The tomato has been a model plant for studying fruit traits…

High latitude and altitude plants experience short growing seasons, consistent low temperatures, windy environments and unpredictable sunny spots. These plants are usually small and rely on self-pollination, but in Campbell, a Sub-Antarctic island, there are gardens of megaherbs that defy the predictions…

The evolution of fruits might have been the most important feature that made angiosperms so successful. Fruits protect seeds and contribute to seed dispersal, as well as comprise a food source for humans and other animals, therefore, studying their evolution would help us shed light on the evolution…

Tomatoes have an indeterminate growth pattern that arises from the balance of flower-promoting (florigen) and flower repressing activities. Suppressing this flower-repressing activity leads to greater determinacy and facilitates cultivation. Soyk et al. compared flowering times in short- and long-days…

Light is good, too much light is bad. Photosynthetic organisms can dissipate excess light through a variety of means including non-photochemical quenching. Czechowski et al. show that in Chlamydomonas perception of UV-B light via the nuclear/cytosolic receptor UVR8 induces accumulation of proteins that…

A pair of reviews in J. Exp. Bot. covers aspects of seed dormancy and germination. Steinbrecher and Leubner-Metzger (10.1093/jxb/erw428) provide an excellent introduction to materials science including stress-strain curves and Young’s Modulus, which they then apply to an understanding of the biophysics…

The Common Symbiosis Signaling Pathway (CSSP) conveys the perception of endosymbionts (rhizobia or mycorrhizal fungi) at the plasma membrane to the nucleus to initiate transcriptional responses. Calcium oscillations are core to the CSSP, whether the endosymbiont is fungal or bacterial. Barker et al.…

Flowers use a variety of strategies to attract pollinators and ensure successful pollination, including color and scent. Moyroud and Glover review some of the less familiar strategies including physical alterations to reflected light. As one example, conical epidermal cells on snapdragon flower petals…

A germinated pollen grain extends a pollen tube through the stigma and style of the flower to deliver two sperm cells to an ovule.  Tip-directed growth of the pollen tube requires a large energy input, but how does the pollen tube obtain energy while growing through the flower tissues?  Goetz et al.…

Animals can easily establish associations between environmental cues and food sources, acquiring conditional information that guides their foraging behaviour and in consequence, their survival. Proving whether plants show association or conditional learning has been tricky, but fortunately for us, Gagliano…

Self-fertilization in Primula is avoided by the production of two flower forms (morphs), one with a long style in which the stigma is elevated above the anthers (the L morph or pin) and one with a short style in which the anthers are well above the stigma (the S morph or thrum), although there are also…

Chlorophyll has an aliphatic phytol side chain that anchors it to light-harvesting complexes. During senescence, chlorophyll is degraded first by the enzymatic removal of Mg to produce pheophytin, which is dephytlated by pheophytinase. Through the identification of a mutant allele with elevated enzymatic…

Terrestrial plants are hosts to diverse types of microbes, predominantly bacteria, that affect plant health and growth in numerous ways. The major types of plant microbiota include plant pathogens, arbuscular mycorrhizal (AM) fungi, endophytes (residing within plant tissues), epiphytes (residing on plant…

Matt and Umen introduce the multicellular green alga Volvox carteri as a model for developmental studies. They provide an overview of embryonic patterning including the role of asymmetric cell divisions, inversion, (a process with some similarities to vertebrate gastrulation), and the role of cell size…

The transition between vegetative and reproductive stages in the plant life cycle implies a change in the developmental program of the shoot apical meristem to stop developing leaves and start developing floral buds. The factors that allow this transition to happen are many and the underlying mechanisms…

IN BRIEF by Jennifer Mach [email protected] Green may have been the Pantone Color of the Year for 2013 (http://www.pantone.com/color-of-the-year-2013), but 2016 was a great year for papers on chlorophyll research, at The Plant Cell and beyond. In this year, we saw a pile of interesting papers examining…

In Plant Physiology Preview you can get a head start on reading the excellent set of articles from a forthcoming special issue on Pollen Biology. Updates and research articles cover all aspects of this crucial part of reproductive biology, from the complex cell biology that underpins polar growth of…

We’ve highlighted some of the Plant Cell papers that were widely shared, liked, blogged, retweeted and otherwise garnered high-levels of attention this year. Perhaps you can use some holiday-season quiet time to catch up on those you missed. Reviews and Perspectives Creating order from chaos: epigenome…

IN BRIEF by Jennifer Lockhart [email protected] Most plants are hermaphrodites, producing perfect flowers with both male and female functions. In roughly 6% of plants, however, male (usually XY) plants produce only male flowers and female (XX) plants produce only female flowers. These dioecious plants…

We’ve highlighted some of the Plant Physiology papers that were widely shared, liked, blogged, retweeted and otherwise garnered high-levels of attention this year. Perhaps you can use some of that holiday-season quiet time to catch up on those you missed. The breakaway attention-getter from Plant…

Farming mutualisms, in which an organism benefits from another to promote growth, have evolved in many lineages. In particular, symbioses between plants and ants are mostly defensive mutualisms. In this paper, Chomicki and Renner describe the obligate mutualism observed between epiphytes in the genus…

Pitcher plants are carnivorous, similar to Venus fly traps.  However, pitcher plants have fluid-filled modified leaves instead of the movable lobes found on Venus fly traps.  The pitcher plant’s modified leaf contains bacteria-laden water that traps small insects, but how are the insects trapped…

HAK5 is a high-affinity potassium transporter that is transcriptionally repressed in high K+ conditions. Zhao et al. identified hormone-related cis-elements in the HAK5 promoter. They screened mutants deficient in transcription factors associated with these cis-elements and found that arf2 mutants show…

Questions remain about the evolutionary origins and functions of stomata. They are absent from liverworts, present to a limited extent in mosses, and are found on 410 million year-old fossils of Cooksonia, a leafless plant. Chater et al. show that orthologs of two key transcription factors that control…

Plant cells detect gravity as a consequence of the movement of dense starch granules called statoliths when the statoctyte, the cell that encompasses, them reorients. An open question has been whether the position of the statoliths within the statocyte or the force exerted by them is the primary gravisensing…

Rubisco (Ribulose-1,5-bisphosphate carboxylase / oxygenase) is the enzyme responsible for fixing almost all inorganic carbon into organic form, but it is not optimized for current conditions. As temperature and CO2 levels increase, there is an opportunity to increase photosynthetic efficiency by engineering…

Gibberellin hormones were famously identified as the product of Gibberella fujikuroi, a fungal pathogen that stimulates host cell elongation, and then subsequently recognized as a hormone produced by plants as well. Fungi and plants produce gibberellins from distinct biochemical pathways, in an example…

Earth colonization by life happened billions of years ago. Weathering of soils by microbial mats leave a characteristic signal that can be used to shed light on the mechanisms involved in colonization. In this review, Lenton and Daines discuss the growing mass of evidence that points to the biogeochemical…

Cells recognize invaders through both cell-surface receptors and intracellular receptors, the latter of which can recognize the invader directly or indirectly, for example through its effects on host proteins. Intracellular surveillance proteins in animals and plants share a core domain, the nucleotide…

Shoot branching increases the photosynthetic surface area and the points at which reproductive structures can form. In angiosperms, auxin (specifically, auxin depletion) has been shown to be involved in the initiation and outgrowth of shoot branches. For example, in apical dominance the primary shoot…

"The endosperm is often viewed as a complicated and rather strange tissue" begins this review by Gehring and Satyaki. They go on to describe that the endosperm is the site of expression of imprinted genes, which are genes that are expressed soley when inherited from the mother or father.  The authors…

Biofortification is the nutritional enhancement (using conventional or genetic engineering approaches) of food with vitamins or micronutrients with the goal of improving the human diet. A set of new reviews in Current Opinion in Biotechnology summarizes progress towards biofortification of plants to…

The cytosol of most plant cells are connected by plasmodesmata, tiny channels that form bridges between adjoining cells (guard cells notably lack plasmodesmatal connections). Water, ions, small molecules, proteins and viruses can move through plasmodesmata. Upon wounding or infection, plasmodesmatal…

The Plant Biotic Interactions (PBI) program supports research on the processes that mediate beneficial and antagonistic interactions between plants and their viral, bacterial, oomycete, fungal, plant, and invertebrate symbionts, pathogens and pests. This joint NSF-NIFA program supports projects focused…

The Arnold Arboretum of Harvard University promotes and supports research consistent with its mission to discover and disseminate knowledge of the plant kingdom. To foster both independent and collaborative work, the Arboretum offers fellowships and awards to students, post-doctoral researchers, and…

The U.S. Department of Energy's Office of Science, Biological and Environmental Research (BER), and the U.S. Department of Agriculture (USDA), National Institute of Food and Agriculture (NIFA), hereby announce their interest in receiving applications for genomicsbased research that will lead to the improved…

Our understandings of the forces that have shaped Earth and the forces that have shaped life on Earth have common roots. Charles Darwin was famously inspired by the work of early geologists such as Charles Lyell, who proposed that Earth was subject to slow but gradual change. This idea recurs in Darwin’s…

Slides from the recent CRISPR/Cas workshop held at the GARNet2016 meeting are now available to download. Organized by Vladimir Nekrasov and Amanda Hopes (The Sainsbury Laboratory/University of East Anglia, UK), the workshop title was, "Introduction to CRISPR-Cas, troubleshooting target design…

From Science, By Jon Cohen Sep. 7, 2016 http://www.sciencemag.org/news/2016/09/did-swedish-researcher-eat-first-crispr-meal-ever-served In what Swedish plant scientist Stefan Jansson declares “maybe” a historic event, he cultivated, grew, and ate a plant that had its genome edited with CRISPR-Cas9.…

Plant pathologists like all other scientists are imparted scientific knowledge and technical expertise, building the educational side of their career. Beckerman and Schneider emphasize the pressing needs of teaching ‘soft skills’ scientists need in order to improve their leadership and professional…

It has been stated that adaptive evolution occurs in plant populations with respect to various complex traits. Olofsson et al. validated the hypothesis that different components of a complex trait can evolve in isolation and later be combined by gene flow by using C4 photosynthesis as a study system.…

Crassulacean acid metabolism (CAM) is a specialized form of photosynthesis that greatly increases water-use efficiency by taking up CO2 through stomata that are open at night (when evapotranspiration is low). Engineering plants that can switch to CAM during periods of drought is a key goal towards…

Traditionally, the production of hybrid maize seed involved the removal of male flowers to prevent self-fertilization of the female flowers. Rice produces bisexual flowers, so mechanical emasculation is not as amenable. Therefore, hybrid rice production requires that the female parent be genetically…

Although it is widely accepted that high humidity has a strong influence on plant diseases of the phyllosphere (the above-ground portions of the plant), the molecular basis is not understood.  Xin et al. report that an important step in bacterial infection of the phyllosphere is a pathogen-driven…

When villagers see the marauding hordes approaching, they secure their food sources. Similarly, when the cell-surface FLS2 receptor detects a bacterial pathogen, it (through its co-receptor BAK1) phosphorylates and stimulates the activity of a cell-surface sugar transporter (STP13), leading to the…

The wavelenghts of light perceived by a plant are information-rich, and plants integrate information from photoreceptors tuned to different wavelenghts to optimize their growth and development. Because plants absorb red light but not far-red light, a low ratio of red to far-red light indicates vegetative…

A seedling in the dark produces ethylene, which in a dicot such as Arabidopsis leads to apical hook formation that protects the cotyledons from damage as the seedling pushes through the soil. The emergence of the seedling into the soil causes a rapid transition to photomorphogenesis and a suppression…

Seed vitality is critical for plants’ evolutionary survival and food production by economic crops such as rice, wheat, etc. Seed formation is preceded by well-coordinated events involving mainly fertilization, endosperm and seed coat formation in chronological order. How is the signal relayed from…

Youssef et al. investigate how the vsr2 mutant affects inflorescence (spike) development in barley. In the two-row variety, at each node there are two sterile spikelets and a single grain forms. In the six-row variety, each of the spikelets is fertile and three grains form at each node. The vsr2 mutation…

A metabolon is a physical clustering of metabolic pathway proteins. Until now, evidence for metabolons has been patchy, but Laursen et al. convincingly demonstrate the physical association of several enzymes involved in the production of dhurrin, a defense compound of sorghum. They use a method involving…

Nitrogen-fixing cyanobacteria such as Anabaena sp. PCC 7120 have the challenge of supporting nitrogenase, an enzyme that is highly sensitive to oxygen, and simultaneously photosynthesis, an oxygen-producing set of reactions. They accomplish this by segregating these reactions into two cells, heterocysts…

The yield of a maize plant is correlated with the size, shape and number of kernels it produces. Miller et al. have developed an automated system to quantify these crucial attributes from photographs of kernels both on and off the cob. Their methods will enable researchers and breeders to record…

Unravelling the mechanism and signals originating from roots and able to contribute to stomatal control has been the subject of research for decades. In this commentary, Tardieu summarizes and discusses many studies and models on root-shoot signals, including work in the same issue by Visentin et…

In an interesting and accessible review, Peyraud et al. provide a big-picture view of how systems biology can be used to address plant-pathogen interactions. They describe the core interactions scaling from molecular to ecosystem / environment, and describe the different types of models that can…

Smell is one of the many senses that insects use to locate the plants that they use as food sources. Plants have evolved ways to exploit insects’ sense of smell, for example by producing volatile compounds that attract pollinators. Pickett and Khan review plant volatile–mediated signals in terms…

Programmed cell death (PCD) is an active process that occurs as part of normal development and also contributes to defense against pathogens. While there are many similarities in developmental PCD (dPCD) and pathogen-triggered PCD (pPCD), there are also differences. Huysmans et al. review and contrast…

Svitashev et al. demonstrate a new way to introduce CRISPR-Cas9 reagents into a plant cell, through the biolistic delivery of pre-assembled Cas9-gRNA ribonucleoproteins. This approach was successful in the production of engineered plants, and also demonstrated that this method can be done in a DNA- and…

Determining the exact phenotypes of a large population of plants, including architectural parameters, metabolic status, and developmental stage, has traditionally required substantial, expensive labor by legions of workers. While technologies for high-throughput genotyping have mushroomed, technologies…

IN BRIEF by Kathleen L. Farquharson [email protected] Alternative splicing (AS) regulates gene expression and greatly expands the coding capacity of complex genomes. By regulating which elements of an mRNA transcript are removed or retained, AS produces multiple transcripts from a single gene. Some…

IN BRIEF by Nancy R. Hofmann [email protected] A new study calls into question whether phytochrome B (phyB) must directly interact with phytochrome-interacting factors (PIFs) to promote light responses. Phytochrome photoreceptors mediate responses to red light in part by inducing the degradation of…

Recently, we’ve been profiling first authors of Plant Cell papers that are selected for In Brief summaries. Here are the first-author profiles from the October issue of The Plant Cell. Jaewook Kim, Kijong Song, and Eunae Park, featured authors of Epidermal Phytochrome B Inhibits Hypocotyl Negative…

As of January 2017, The Plant Cell will offer authors the option of associating a Peer Review Report with each research article. Reviewer anonymity will be strictly maintained. The reports will include the major comments from reviewers and the editors’ decision letters along with the authors’ response…

IN BRIEF by Gregory Bertoni [email protected] RNA metabolism is key to a number of crucial processes in the cell, including transcription, RNA splicing, translation, and gene regulation. For efficient translation, mature mRNAs must have a 7-methylguanosine cap on the 5′ end to help recruit the translation…

Recently, we’ve been profiling first authors of Plant Cell papers that are selected for In Brief summaries. Here are the first-author profiles from the October issue of The Plant Cell. Olivia Wilkins and Christoph Hafemeister, featured first authors of EGRINs (Environmental Gene Regulatory Influence…

Warmest congratulations to Yoshinori Ohsumi, 2016 Nobel Laureate in Physiology or Medicine, “for his discoveries of mechanisms for autophagy”.  Autophagy (self-eating) is a process through which cells selectively degrade and recycle cellular components. Ohsumi’s research has primarily focused…

IN BRIEF by Nancy R. Hofmann  [email protected] Plant cell walls consist of cellulose microfibrils embedded in a matrix of polymers including hemicelluloses. As one of the main hemicelluloses in the cell walls of dicots, xyloglucan is an important target of study to understand plant cell walls…

IN BRIEF by Jennifer Mach [email protected] Plants can dispose of organs such as leaves and recycle the nutrients in these organs into new leaves, seeds, or storage organs. However, when separated from its photosystem proteins, chlorophyll can be phototoxic, absorbing light and producing high-energy…

IN BRIEF by Jennifer Lockhart [email protected] Heat and drought stress greatly restrict crop productivity, but most of what we know about a plant’s response to these stresses comes from controlled laboratory studies. This factor, along with the complex nature of these responses, has hampered efforts…

IN BRIEF by Nancy Hofmann [email protected] The ability to generate transgenic plants without regard to cultivar or genotype can be considered a holy grail of cereal crop transformation. Despite years of effort, it has been remarkably difficult to develop efficient methods for transformation of…

Recently, we’ve been profiling first authors of Plant Cell papers that are selected for In Brief summaries. Here are the first-author profiles from the September issue of The Plant Cell. Inmaculada Couso, featured first author of Synergism between inositol polyphosphates and TOR kinase signaling…

Recently, we’ve been profiling first authors of Plant Cell papers that are selected for In Brief summaries. Here are the first-author profiles from the August issue of The Plant Cell. Aman Y. Husbands and Vasudha Aggarwal, featured first authors of In Planta Single-Molecule Pull-down (SiMPull)…

IN BRIEF by Jennifer Mach [email protected] Assessing protein-protein interactions remains a fundamental challenge for plant biologists. Current methods such as coimmunoprecipitation (co-IP), yeast two-hybrid, bimolecular fluorescence complementation (BiFC), and others can produce artifacts and also yield…

Carotenoids are nutritionally important phytonutrients. Comas et al. describe a strategy to enhance the production of cartotenoids in the seed endosperm. They start with quantitative metabolomics and gene expression data which they feed into mathematical models to determine which gene(s) need to be engineered.…

Recently, we’ve been profiling first authors of Plant Cell papers that are selected for In Brief summaries. Here are the first-author profiles from the July issue of The Plant Cell. Fangwei Gu, featured first author of Arabidopsis CSLD5 functions in cell plate formation in a cell cycle-dependent manner Current…

IN BRIEF by Kathleen L. Farquharson [email protected] Polysaccharide-rich cell walls are a distinguishing feature of plants that influence many aspects of growth and development, including cell division. Whereas contractile rings pinch dividing cells into two daughter cells in other eukaryotes,…

IN BRIEF by Nancy Hofmann [email protected] Most bacteria have a peptidoglycan layer between the inner and outer membranes (reviewed in Typas et al., 2012). The cyanobacterial endosymbiont that gave rise to plastids would have contained such a peptidoglycan wall including d-amino acids. Indeed, peptidoglycan…

Shih et al. review how the many and diverse tools of plant synthetic biology can be applied towards bioenergy crops, focusing on traits related to lignocellulose, oil, and soluble sugars. Tools include those that edit genes, those that alter protein activities, and those that enable gene stacking in…

IN BRIEF by Jennifer Lockhart [email protected] When a deer eats the primary shoot of a plant, this can activate a nearby dormant axillary bud, causing it to form a secondary shoot. Genetic and environmental factors also affect shoot architecture, which strongly influences crop productivity. Changes…

IN BRIEF by Jennifer Mach [email protected] Movement of macromolecules through the plant phloem provides a mechanism for long-distance signaling that plants use in development, disease resistance, and other adaptive responses (reviewed in Spiegelman et al., 2013). For example, full-length RNAs, such…

IN BRIEF by Jennifer Lockhart  [email protected] Investigating how plants grow and develop often requires a bit of creativity. For example, deep within the plant, the vascular cambium, a layer of embryonic, highly cytoplasmic cells, gives rise to xylem and phloem tissue, which must expand throughout…

Nemhauser and Torii define synthetic biology as “an engineering approach to design, build and analyize dynamic molecular devices and/or pathways from biological components to produce cells and organisms with customized functionality.” In their review, they describe several plant synthetic biology…

Many plant traits are multigenic, so engineering them requires modulating the expression of several genes simultaneously. Synthetic promoters and transcription factors offer such a possibility. For example, a cis-element can be introduced into the promoter of each gene of interest, and a synthetic transcription…

Raitskin and Patron review efforts to express multiple single guide RNA (sgRNAs) and Cas9 in plants for the coordinated expression of many genes. They argue for the need to create single plasmids carrying the sgRNAs and Cas9, using a Type IIS restriction endonuclease-mediated assembly method. Curr. Opin.…

Puchta reviews the different ways that CRISPR/Cas9 can be used in synthetic biolgy, beyond the common gene-editing function. For example, the Cas9 protein can be fused to other proteins that activate or repress transcription, and targeted to different promoters using guide RNAs. He further explores the…

Control of gene expression involves both activation and repression of transcription. Schaumberg et al. used transient expression in Arabidopsis and sorghum protoplasts and dual-luciferase outputs to rapidly quantitate synthetic repressors and repressible promoters, and verified their results in transgenic…

Patron et al. describe the need to standardize DNA parts and terminology, so that researchers and inventors can use off-the-shelf parts (they draw an analogy to the standardization of components in mechanical and electronic engineering). New Phytol. 10.1111/nph.13532