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Protein-protein interactions are crucial to our understanding of biology but can be hard to detect. Trigg et al. developed a sophisticated yeast two-hybrid assay augmented with Cre recombinase (CrY2H-seq) to identify the Arabidopsis transcription factor protein-protein interactome.  In this method,…

Plants, green algae and cyanobacteria carry out oxygenic photosynthesis through the coordination of two photosystems, PSI and PSII. Many other photosynthetic prokaryotes use a single reaction center to carry out anoxygenic photosynthesis. Gisriel et al. describe the structure of a photosynthetic reaction…

Long et al. examined transcription factor complex formation in vivo in Arabidopsis roots using a technique that combines FRET (Förster Resonance Energy Transfer) and FLIM (Fluorescence Lifetime imaging Microscopy). Using this method, the authors were able to observe cell-type specific complex formation…

Pigment engineering was featured in three recent papers. Anthocyanins are blue pigments valued for their antioxidant health benefits and for their beauty, but their biosynthesis and chemistry is complex. Noda et al. introduced two genes to produce blue anthocyanins in chrysanthemum petals (Sci Advances…

Saffer et al. identified an Arabidpsis mutant with swirled petals and with petal epidermal cells that show a left-handed (but never right-handed) twist. They mapped the mutation to the RHAMNOSE BIOSYNTHESIS1 (RHM1) gene, which is most highly expressed in petal epidermal cells and encodes an enzyme that…

It’s easy to demonstrate that plants sense gravity, and we also know that statocytes are involved in the perception of gravity. Statocytes are gravity-sensing cells that contain dense starch-containing amyloplasts that move within the cell in the direction of gravity. Differential growth to accommodate…

Parasitic plants such as Cuscuta astralis (dodder) form connections with their host plants through which nutrients and other molecules pass. Using mutant plants and transcriptomic assays, Hettenhausen and Li et al. showed that two or more plants connected by Cuscuta bridges shared information through…

Plant hormones are made in one tissue and usually transported to act in another. One example of this is the transport of cytokinin.  In the root, the precursor trans-zeatin riboside (tZR) is synthesized, then xylem loaded and transported to the shoot. Once at a site of action like the leaf or shoot…