Entries by Rachel Shahan

Father Knows Best? Small RNA Pathway Controls Endosperm Response to Paternal Genomic Dosage

In flowering plants, seed viability is dependent upon the endosperm, a triploid tissue produced by fertilization of a diploid central cell by a haploid sperm. Most endosperm genes are expressed in a 2:1 maternal to paternal ratio reflecting the genomic DNA content. Consequently, balance between maternal and paternal genome dosages is important for proper endosperm, […]

The Future is Now: Gene Expression Dynamics at Single Cell Resolution

For the past 10 years, a popular method to study global gene expression changes has been RNA sequencing of bulk tissues. However, an inherent limitation of this approach is the confounding of multiple cell types or multiple developmental stages. By contrast, new technologies enable transcriptional profiling of thousands of cells at single cell resolution. Single […]