Antimicrobial solid media for screening non-sterile Arabidopsis thaliana seeds (bioRxiv)
Transgenic plant production often involves an agar-based selection step in which plants that have taken up the introduced DNA (containing a selectable marker) show resistance to a corresponding antibiotic. Typically, putatively transformed seeds are surface sterilized prior to plating so that microbes don’t outgrow the much slower-growing plants. This step is time consuming and can […]