Recent Posts

Nanoscale movements of cellulose microfibrils in primary cell walls ($)

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Cell walls are complex mixtures of cellulose microfibrils, proteins and other materials. Their mechanical properties can be measured and modeled, but it is not always simple to translate these measurements to changes at the molecular level. Zhang et al. used atomic force microscopy to provide an unprecedented…

Design principles of a minimal auxin response system (Nature Plants)

Auxin controls virtually all facets of growth and development. This plant hormone is sensed by TIR1/AFB F-box receptors which promote ubiquitin-mediated degradation of AUX/IAA transcriptional repressors, releasing ARF transcription factors from inhibition. Phylogenetic analyses divide the ARF family…

Flippases Function in PIN Polarity

Zhang et al. elucidate the role of flippases in the polarity and trafficking of PIN auxin transporters. Plant Cell https://doi.org/10.1105/tpc.19.00869 By Xixi Zhang and Jiří Friml Institute of Science and Technology Austria (IST Austria), Am Campus 1, 3400 Klosterneuburg, Austria  Background:…

Auxin response factor targeted by viruses (PNAS)

Auxin regulates various aspects of plant growth and development and it also contributes to plant defense. Auxin activates downstream signaling by promoting degradation of the repressor Aux/IAA proteins to liberate the key transcription factors ARFs (auxin response factors). Pathogens have been known…

An Improved Recombineering Toolset for Plants

Brumos and Zhao et al. developed an improved set of genetic tools to make precise sequence modifications in large DNA fragments and used them to tag hundreds of plant genes, including those involved in auxin production. Plant Cell https://doi.org/10.1105/tpc.19.00431 By Javier Brumos, Anna Stepanova,…

A Simple and sensitive SYBR Gold-based assay to quantify DNA-Protein interactions ($) (Plant Molecular Biology)

Gene regulation occurs at multiple levels including transcription. During transcription, transcriptional factors bind to specific target sequence which allows the recruitment of other proteins including RNA polymerase that enables transcription. Understanding such an intricate mechanism allows researchers…

Fine control of aerenchyma and lateral root development through Aux/IAA- and ARF-dependent auxin signaling ($) (PNAS)

Auxin plays a major role in plant development and can alter the developmental program following the stress response. In this paper, Yamauchi et al., have identified the auxin signaling cascade involved in the development of aerenchyma and regulators of this auxin signaling might also be involved in lateral…

Auxin-sensitive AUX/IAA proteins mediate drought tolerance in Arabidopsis by regulating glucosinolates levels ($) (Nature Communication)

Glucosinolates are secondary metabolites synthesized by plants as a defense compound against pathogen and herbivore. In this paper Salehin et al., have demonstrated regulation of aliphatic glucosinolate levels by auxin signaling that promotes drought tolerance in Arabidopsis. The Aux/IAA proteins are…

Flexibility of intrinsically disordered degrons in AUX/IAA proteins reinforces auxin receptor assemblies ($) (BioRxiv)

Auxin is involved in multiple plant development and stress response which necessitates complexity in auxin signaling. Auxin at a particular threshold in the cells brings together the TIR1/AFB1-5 (TRANSPORT INHIBITOR RESPONSE1/ AUXIN SIGNALING F-BOX 1-5) members and members of the 29 Aux/IAA members bound…

Design principles of a minimal auxin response system ($) (BioRxiv)

Auxin signaling components are evolutionarily conserved across the land plants and the duplication events in these regulators enabled complexity in development. Auxin regulated developmental programming occurs through the ARF (AUXIN RESPONSE FACTORS) transcriptional factors. Marchantia polymorpha serves…