Review: The increasing impact of activity-based protein profiling in plant science

Activity-based protein profiling is a proteomics approach that involves covalently labeling reporter tags to subsets of proteins based on their active sites. Morimoto and van der Hoorn define different types of probes and the types of proteins that they bind to. Activity profiling can reveal different activity levels of closely related protein-family members, and their differential responses to pathogens or stimuli. It can also reveal differential inhibitor effects. The authors describe several useful activity probes and their targets, and show how activity-based protein profiling has already provided insights into pathogen responses and developmental processes. Plant Cell Physiol. 10.1093/pcp/pcw003