Review: Multi-gene engineering in plants with RNA-guided Cas9 nuclease ($)

cobiotechcas9Raitskin and Patron review efforts to express multiple single guide RNA (sgRNAs) and Cas9 in plants for the coordinated expression of many genes. They argue for the need to create single plasmids carrying the sgRNAs and Cas9, using a Type IIS restriction endonuclease-mediated assembly method. Curr. Opin. Biotech. 10.1016/j.copbio.2015.11.008