Recognizing Plant Physiology author Haixin Long

Haixin Long, co-first author of Narrow Leaf21, Encoding Ribosomal Protein RPS3A, Controls Leaf Development in Rice

Current Position: R&D Engineer, Coyote Bioscience Co., Ltd.

Education: MSc at Institute of Crop Sciences, Chinese Academy of Agricultural Sciences

Non-scientific Interests: Yoga and travelling

Brief Bio:

In 2013, I entered the laboratory of Professor Xueyong Li, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences for a master’s degree, engaged in the research on the genetic and molecular mechanism of rice leaf width. My research mainly focuses on rice narrow-leaf mutants. In this study, we screened a rice narrow leaf mutant nal21 by chemical-induced mutagenesis, and cloned the candidate gene NAL21 by map-based cloning approach. In the nal21 mutant, the 5′-splicing site of the second intron of NAL21 was mutated from GT to AT, leading to mis-splicing of the second intron. Amount of the 40S small ribosomal subunit was reduced, and translation efficiency of many auxin response factors (such as OsARF11, OsARF16) and the key transcription factor regulating leaf blade lateral expansion OsWOX3A was decreased, resulting in the narrow leaf phenotype in the nal21 mutant. This study is very helpful to improve the molecular regulatory network of rice leaf width development and provides guidance for rice plant type breeding.

姓名：龙海馨

职位：卡尤迪生物科技（北京）有限公司 研发工程师

教育经历：中国农业科学院 作物科学研究所，硕士

兴趣爱好：瑜伽，旅行

个人简介：

我于2013年进入中国农业科学院作物科学研究所李学勇老师实验室进行硕士学习，从事调控水稻叶片宽度的分子遗传机理研究。我的课题研究主要围绕水稻窄叶突变体，在该研究中我们通过化学诱变筛选到一个水稻窄叶突变体nal21，并图位克隆获得了候选基因NAL21。NAL21基因编码核糖体小亚基蛋白RPS3A，在 nal21突变体中该基因第二内含子5’端拼接点处由GT突变为AT，导致拼接错误。在nal21突变体中，40S核糖体小亚基数量显著减少，多个生长素响应因子（如OsARF11，OsARF16）和调控叶片侧向生长的关键转录因子OsWOX3A的蛋白翻译水平降低，最终导致窄叶叶型的出现。该研究有助于完善水稻叶片宽度发育的分子调控网络，对水稻株型育种具有重要的指导意义。