In vivo FRET–FLIM reveals cell-type-specific protein interactions in Arabidopsis roots ($)

Long et al. examined transcription factor complex formation in vivo in Arabidopsis roots using a technique that combines FRET (Förster Resonance Energy Transfer) and FLIM (Fluorescence Lifetime imaging Microscopy). Using this method, the authors were able to observe cell-type specific complex formation between transcription factors known to specify root development, specifically, SHORT-ROOT (SHR), SCARECROW (SCR).  JACKDAW (JKD), and NUTCRACKER (NUC). The authors found that protein complexes can change their conformation in a cell-type dependent manner to regulate gene expression leading to precise specification and maintenance of particular cell fates within the Arabidopsis root meristem. Nature 10.1038/nature23317