High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell
The current version of the Arabidopsis thaliana Col-0 reference genome, TAIR10, still has some gaps and mis-assemblies due to centromeres and repeat-rich regions. In another demonstration of the promise of single-molecule sequencing, Michael, Jupe et al. used a single nanopore flow cell to sequence the Arabidopsis thaliana accession KBS-Mac-74 in “four days at a cost of less than 1,000 USD for sequencing consumables including instrument depreciation.” bioRxiv 10.1101/149997
