Cytokinin signaling patterns maize leaves, otherwise things get hairy and frayed
Variation in leaf morphology across the angiosperms is impressive and abounds. Leaves are the primary photosynthetic organ for most angiosperms, acting as the primary sites of light capture and gas exchange. Typical of grass leaves, maize leaves are strap-like and simple, with unelaborated margins. Leaves take their shape early in development, arising as lateral primordia at the flank of the shoot apical meristem. As the primordium emerges from the meristem periphery, the three distinct axes that will define its mature form are established: proximal/distal (base/tip), medial/lateral (middle/margin) and ad/abaxial (upper/lower) (Conklin et al., 2019). A key feature along the proximal/distal axis of maize leaves is the ligular region, where the auricle and ligule form a striking morphological boundary between the proximal sheath and distal blade. Understanding the genetic basis of leaf patterning is a central topic in plant developmental biology. Here, Muszynski et al. (2020) describe the role cytokinin plays in establishing a proximal/distal axis during maize leaf development.
The semi-dominant mutation Hairy Sheath Frayed1 (Hsf1) has captured the attention of maize geneticists for nearly thirty years (Bertrand-Garcia and Freeling, 1991). Plants heterozygous for Hsf1 exhibit mutant leaves with marginal outgrowths, or “prongs,” that fully recapitulate the blade/sheath boundary along the leaf margin (see Figure), as well as dense macrohairs along the abaxial sheath (hence the mutant’s name). Muszynski et al. (2020) showed that prongs form in certain domains of the blade, with larger prongs appearing more proximally, suggesting that prong initiation is developmentally regulated. To understand the genetic basis of the Hsf1 phenotype, the authors used map-based cloning to identify independent causative mutations in a gene encoding maize HISTIDINE KINASE1 (ZmHK1), a putative cytokinin receptor. Interestingly, all Hsf1 alleles have missense mutations encoded in the ZmHK1 receptor CHASE domain, a highly conserved stretch of amino acids with demonstrated binding affinity for various cytokinin ligands (Hothorn et al., 2011).
With the candidate gene in hand and given the CHASE domain harbored amino acid substitutions in Hsf1, Muszynski and coworkers hypothesized that Hsf1 phenotypes could be due to cytokinin hypersignaling from the ZmHK1 receptor. Using a powerful heterologous histidine kinase signaling assay in yeast, Muszynski et al. (2020) showed that mutant ZmHK1 receptors actively signaled in the absence of cytokinin. Binding assays with the different mutant ZmHK1 receptors expressed in bacterial spheroplasts or tethered within tobacco membranes via transient in planta expression established that missense mutations in the Hsf1 CHASE domain increased the relative binding affinity of ZmHK1 for naturally occurring cytokinin ligands. Modeling the effects of mutations in ZmHK1 on the solved crystal structure of Arabidopsis thaliana AHK4 (Hothorn et al., 2011) suggested that the mutated residues reside near, but not in, the cytokinin binding pocket and may impact the binding affinity for the hormone.
With the Hsf1 leaf phenotypes likely the result of hypersignaling of cytokinin, the authors asked if exogenous application of a synthetic cytokinin could 1) phenocopy Hsf1-related leaf patterning in wild-type seedlings and 2) enhance the leaf phenotypes of Hsf1 mutants. Wild-type seedlings treated with exogenous cytokinin had smaller leaves with dense macrohairs and prongs that developed on the blade margins. In the presence of exogenous cytokinin, Hsf1 seedlings showed enhancement of leaf these phenotypes, demonstrating that Hsf1 was hypersensitive to the hormone. In support of these observations, Muszynski et al. (2020) used qRT-PCR and RNA in situ hybridization to show that the cytokinin type-A response regulator ZmRR3 is ectopically expressed in the shoot apices of plants heterozygous for Hsf1, particularly in the primordial margins at the presumptive position of prong emergence. Turning to genetics, the authors observed a synergistic interaction between Hsf1 and a null allele of ZmRR3, aberrant phyllotaxy1, suggesting that ZmRR3 dampens cytokinin signaling in the maize shoot. Together, this work sheds light on a key role for cytokinin in programming developmental patterning in maize leaves.
Plant Biology Section, School of Integrative Plant Science
Bertrand-Garcia, R., and Freeling, M. (1991). Hairy-Sheath Frayed#1-O: A systemic, heterochronic mutant of maize that specifics slow developmental stage transitions. Am. J. Bot. 78: 747-765.
Conklin, P.A., Strable., J., Li. S., and Scanlon, M.J. (2019). On the mechanisms of development in monocot and eudicot leaves. New Phytol. 221: 706-724.
Hothorn, M., Dabi, T., and Chory, J. (2011). Structural basis for cytokinin recognition by Arabidopsis thaliana histidine kinase 4. Nat. Chem. Biol. 7: 766-768.
Muszynski, M.G., Moss-Taylor, L., Chudalayandi, S., Cahill, J., Del Valle-Echevarria, A.R., Alvarez-Castro, I., Petefish, A., Sakakibara, H., Krivosheev, D.M., Lomin, S.N., Romanov, G.A., Thamotharan, S., Li, B., and Brugière, N. (2020). The maize Hairy Sheath Frayed1 (Hsf1) mutant alters leaf patterning through increased cytokinin signaling. DOI: https://doi.org/10.1105/tpc.19.00677.