Entries by Tatsuya Nobori

Active DNA demethylation controls defense gene regulation and antibacterial resistance (bioRxiv)

Epigenetic reprogramming, such as DNA methylation changes, has emerged as a crucial regulator of plant defense responses. Homeostasis of DNA methylation is controlled by a balance between methylation and demethylation. ROS1 (Repressor Of Silencing 1) is an Arabidopsis demethylases that is known to be a positive regulator of defense, but little is known about the […]

Coordination of microbe-host homeostasis via a crosstalk with plant innate immunity (Research Square)

In nature, roots are colonized by commensal bacteria that do not seem to influence host traits. Recently, studies showed some commensals can suppress plant immune responses when inoculated to plants alone. However, how such commensal activities are coordinated in the community context is poorly understood. Ma, Niu et al. took a bottom-up approach to explore […]

Real time quantitative imaging of transcriptional activity at the single cell level (bioRxiv)

How plants transcriptionally operate developmental programs and responses to stress in time and space has been an important question in plant biology. Fluorescent protein reporters are commonly used to address this question, but their performance is limited at short timescales (<30 min) before the proteins get matured. Also, expression of fluorescent proteins can be influenced […]

An interactome of plant autophagy proteins and pathogen effectors (Cell Host Microbe) ($)

Autophagy is a regulated process whereby select cytoplasmic components are degraded. It is involved in a variety of biological processes, including defense against pathogens. As such, plant pathogens have evolved mechanisms to target host autophagy machinery to gain virulence. However, we still lack a comprehensive understanding of pathogen molecules that alter plant autophagy pathways. Lal […]

Spatial profiling of immune competence in plant roots (bioRxiv)

Plant roots should prevent immune activation by innocuous soil microbiota, as constitutive immune activation is harmful to plant growth. Previous studies have indicated variable immune competence of different root cell types and developmental zones, but this is currently poorly understood with the fine spatial resolution. Emonet et al. combined cell-type specific expression of the immune […]

Driving factors of disease spread in natural plant-pollinator communities (Nature Ecol. Evol.)

Disease spread within species-rich communities is hard to predict and prevent due to the dynamic transmission of parasites between multiple hosts that change in abundance and composition over time. To better understand the temporal dynamics of bee parasites in communities of plants and pollinators, Graystock and Ng et al. sampled 89 species of flowers and […]

Extracellular proteolytic cascade in tomato activates immune protease Rcr3 (PNAS)

The plant apoplast is a sea of immune-related proteins that facilitate robust defense against pathogens. Rcr3 is a tomato secreted apoplastic protease that contributes to both basal defense and gene-for-gene resistance against pathogens. Rcr3 is activated by the cleavage of its autoinhibitory prodomain, and it has been considered that the catalytic activity of Rcr3 is […]

An optogenetic tool for plants grown in ambient light (Nature Methods)

Optogenetics has revolutionized mammalian biology by its ability to fine-tune gene expression in individual cells by pulses of light. However, its application in plants has been challenging because the white light required for plant growth misfires the system. Ochoa-Fernandez et al. solved this problem by engineered photoreceptors that can repress and induce gene expression driven […]

Plant-herbivore chemical communication decoded (Science)

Chemical communications between species are prevalent in nature. For instance, herbivorous insects can spot their host plants by sensing volatile organic compounds (VOCs) emitted by plants. Despite our knowledge about interactions between individual plant and herbivore species, little is known about how chemical communications are structured and maintained in the ecological context. Zu et al. […]