Entries by Suresh Damodaran

Cell cycle dependent regulation and function of ARGONAUTE1 in plants (Plant Cell) ($)

Regulation of gene expression at transcriptional, translational and post-translation levels is crucial for proper plant growth. Post-transcriptional gene regulation by small RNAs like microRNA (miRNA), siRNA and phasi-RNAs is necessary for meristem maintenance in plants. Unlike in the mammalian system, the role of small RNAs during the cell cycle in meristematic tissue is unknown. In […]

ABCB21 regulates auxin levels in cotyledons, pericycle and leaves (Frontiers Plant Science)

Auxin activity is maintained through regulated biosynthesis, metabolism and transport. In this paper Jenness et al. characterized the physiological significance of a known IAA transporter, ABCB21 (ATP BINDING CASSETTE TRANSPORTER subfamily B member 21) in Arabidopsis. ABCB21 transports shoot derived auxin in the rootward direction, and a transcriptional fusion of ABCB21 regulatory elements fused to […]

An Improved plant toolset for high-throughput recombineering (BioRxiv)

To determine the role of any gene of interest it is necessary to understand the spatiotemporal expression pattern. This is accomplished by tagging the regulatory sequence(s) alone (transcriptional fusion) or regulatory sequence(s) with the coding region (translational fusion) of the gene of interest. In this paper Brumos et al., have developed a recombineering based gene […]

Sphingolipid biosynthesis modulates plasmodesmal ultrastructure and phloem unloading (Nature Plants)

Regulated transport of molecules from source to sink is essential for providing necessary molecules including photoassimilates to the required tissue. This mechanism is crucial for development and plant growth in response to stimuli and it occurs through plasmodesmata. In a previous report, CALLOSE SYNTHASE 3 (CALS3) was identified to be necessary for the permeability of […]

Evolution of vascular plants through redeployment of ancient developmental regulators (bioRxiv)

Land plants (Tracheophytes), utilize the well-developed vascular tissue for conducting water and other nutrients necessary for plant growth. In Arabidopsis and other land plants, the key player of vascular cell division is well characterized and this includes the TMO5-LHW (TARGET OF MONOPTEROS 5 – LONESOME HIGHWAY) heterodimerization, downstream of auxin signaling. Loss of either one […]

Pinstatic acid promoters auxin transport by inhibiting PIN internalization (Plant Physiol)

Auxin transport regulated by directional transporters such as PIN (PIN-FORMED) proteins ensures maintenance of proper auxin levels for growth and development. Plants modulate auxin flow by regulating the localization of PIN through exocytosis and endocytosis allowing recycling of PIN protein within a cell. In search of modulators of PIN internalization during such regulation, Oochi et […]

Genetic and Molecular analysis of trichome development in Arabis alpina $ (PNAS)

Trichomes (plant hairs), arise from epidermal cells of plants. The molecular mechanism involved in the development of trichomes of plants has been well studied in Arabidopsis thaliana. In this paper, Chopra et al.  have identified key regulators of leaf trichome development in Arabis alpina, a species that diverged from A. thaliana about 26-40 million years […]

Application of TurboID-mediated proximity labeling for mapping a GSK3 Kinase signaling network in Arabidopsis (bioRxiv)

In this preprint Kim et al., have provided an optimized method for determining protein-protein interaction in plants using TurboID-mediated labelling.  TurboID is an engineered promiscuous biotin ligase that marks proteins it comes in contact with. Identification of interacting partners of a protein through this approach will aid in deciphering the molecular signaling network. In this […]

The lateral root cap acts as an auxin sink that controls meristem size (Curr Biol)

During root growth, cellular activities need to be coordinated across tissues. Mambro et al. have identified molecular mechanisms involved in root apical meristem maintenance by auxin homeostasis regulation in lateral root cap (LRC) cells. The cytokinin response regulator ARR1 regulates expression of the auxin transporter PIN5 and an auxin conjugating amidoacyl transferase, GH3-17, in LRC. […]