Entries by Suresh Damodaran

One thousand plant transcriptomes and the phylogenomics of green plants (Nature)

With a collaborative effort by multiple laboratories across the world, a database of transcriptomics data has been generated for 1124 species encompassing green plants, glaucophytes, and red algae. This extensive work referred to as onekp (one thousand plant transcriptomes) will aid in researchers to understand the functional diversity of gene families, species relationships among several […]

Transcriptional regulatory framework for vascular cambium development in Arabidopsis roots ($) (Nature Plants)

Plant vascular tissues continue to proliferate post-embryogenesis via the cambium, a lateral meristem. Until recently the transcription factors regulating meristem maintenance and promoting differentiation were still unknown. In this paper, Zhang et al. identified multiple transcription factors using global transcriptomics of cambium-specific tissue harvested using fluorescent activated cell sorting. Using differential analysis, multiple transcriptional factors […]

A Simple and sensitive SYBR Gold-based assay to quantify DNA-Protein interactions ($) (Plant Molecular Biology)

Gene regulation occurs at multiple levels including transcription. During transcription, transcriptional factors bind to specific target sequence which allows the recruitment of other proteins including RNA polymerase that enables transcription. Understanding such an intricate mechanism allows researchers to study the spatio-temporal expression pattern of genes necessary for development or inducing signals during biotic or abiotic […]

Fine control of aerenchyma and lateral root development through Aux/IAA- and ARF-dependent auxin signaling ($) (PNAS)

Auxin plays a major role in plant development and can alter the developmental program following the stress response. In this paper, Yamauchi et al., have identified the auxin signaling cascade involved in the development of aerenchyma and regulators of this auxin signaling might also be involved in lateral root development. Aerenchyma is a gas-filled space […]

Auxin-sensitive AUX/IAA proteins mediate drought tolerance in Arabidopsis by regulating glucosinolates levels ($) (Nature Communication)

Glucosinolates are secondary metabolites synthesized by plants as a defense compound against pathogen and herbivore. In this paper Salehin et al., have demonstrated regulation of aliphatic glucosinolate levels by auxin signaling that promotes drought tolerance in Arabidopsis. The Aux/IAA proteins are coreceptors of auxin and repress the activity of AUXIN RESPONSE FACTOR (ARF) bound to […]

Flexibility of intrinsically disordered degrons in AUX/IAA proteins reinforces auxin receptor assemblies ($) (BioRxiv)

Auxin is involved in multiple plant development and stress response which necessitates complexity in auxin signaling. Auxin at a particular threshold in the cells brings together the TIR1/AFB1-5 (TRANSPORT INHIBITOR RESPONSE1/ AUXIN SIGNALING F-BOX 1-5) members and members of the 29 Aux/IAA members bound to the activator ARF (AUXIN RESPONSE FACTOR) transcriptional factors. This complex […]

Minimal auxin sensing levels in vegetative moss stem cells revealed by a ratiometric reporter ($) (New Phytologist)

Auxin plays multiple developmental roles from embryogenesis to seed development. To understand the mechanism of auxin response in plants, researchers utilize the early-diverging land plant models, Physcomitrella patens and Marchantia polymorpha. In this paper, Thelander et al., have identified a specific reporter of auxin levels for P.patens, PpR2D2 (Ratiometric version of DII-nVENUS to mDII-nTdTOMATO signal). This reporter developed for P. patens similar to […]

Design principles of a minimal auxin response system ($) (BioRxiv)

Auxin signaling components are evolutionarily conserved across the land plants and the duplication events in these regulators enabled complexity in development. Auxin regulated developmental programming occurs through the ARF (AUXIN RESPONSE FACTORS) transcriptional factors. Marchantia polymorpha serves as an ideal system to understand ARF activity owing to its simpler genome complexity. In their previous work, Weijer’s Lab […]

Plant Science Research Weekly: October 18

Guest Editor : Suresh Damodaran I am a postdoctoral research associate in Dr. Lucia Strader’s lab at WUSTL. My primary area of interest is understanding the role of plant hormones in development. I completed my graduate degree in Dr. Sen Subramanian’s lab at SDSTATE.  Twitter: @SureshDamod   Design principles of a minimal auxin response system […]