Entries by Rose McNelly

Discovering and characterizing a protein involved in endosperm starch formation in rice

Rice is a globally important crop, with most of its calorific component coming from starch. Despite its importance, we do not fully understand the mechanism of starch formation in the rice endosperm. To discover novel factors involved in this process, Yan et al. performed a forward genetics screen and identified a FLOURY ENDOSPERM 9 (flo9) […]

Altering the location of starch granules by relocalizing a starch granule initiation protein

Starch granules are synthesized in leaf chloroplasts during the day and degraded at night to provide carbon. MFP1 (MAR-BINDING FILAMENT-LIKE PROTEIN 1) is known to have a role in starch granule initiation, but its mechanistic role has been unknown. Sharma et al. hypothesized MFP1’s thylakoid localization signals are important for its role; to test this […]

A novel starch granule size distribution in Arabidopsis thaliana is associated with differences in phosphorylation

During the day, starch is synthesized in the chloroplasts of leaves and stored as starch granules, before being degraded in the subsequent night. In Arabidopsis thaliana, starch granules are discoid in shape. Some mutants have round or elongated granules, yet all these mutants have a homogenous starch granule morphology. Here Li et al. generated Arabidopsis […]

A novel way to conduct genome wide association studies for secondary metabolites

Plants produce many specialized metabolites that are often associated with increased plant fitness. Metabolic genome wide association studies (mGWAS) identify enzymes involved in specialized metabolite biosynthesis. However, this relies on identifying metabolites from mass spectrometry data which is often time and labor intensive. Here, Zhu et al. bypassed this step by identifying structural modifications rather […]

Review: Increasing yields of barley and wheat through inflorescence architecture

The Triticeae is an important tribe of crops that contains both barley and wheat. As the global population increases, the yield of Triticeae crops must increase to meet global food demands. An important factor influencing yield is grain number per spike, which is affected by inflorescence architecture. Here, Zhang et al. suggest that altering the […]

Subsidiary cells mediate stomatal closure in maize

Stomata are pores on leaves which enable gas exchange. In grasses, stomata are surrounded by dumbbell-shaped guard cells that are flanked by subsidiary cells. However, the role of the subsidiary cells in stomatal closure is not fully understood. Here, Liu et al. investigated this using two maize (Zea mays) mutants, called pangloss1 (pan1) and pangloss2 […]

Altering chloroplast biogenesis leads to increased yields in rice

Transcription of chloroplast genes is carried out by the plastid encoded polymerase (PEP) with help from PAPs (PEP-associated proteins). PAP3 is important for chloroplast development in Arabidopsis thaliana and Nicotiana benthamiana, however its role in crops has not been fully elucidated. Here Seo et al. transformed rice to express OsPAP3 under the constitutively active 35S […]

Staining starch granules in living plants

Starch granules are synthesized in the leaves of chloroplast during the day and degraded in the subsequent night. To visualise starch granules the tissue usually has to be fixed, which prevents live imaging. Here Ichikawa et al. set out to find a highly specific dye to image starch granules in living tissues. They tested a […]