Targeted A-to-G base editing of chloroplast DNA in plants
The chloroplast has its own genome which encodes proteins needed for chloroplast function. Editing these genes via CRISPR-Cas9 is difficult due to challenges in targeting guide RNAs to the chloroplast. Instead, base editors can be used, but these were originally developed for cytosine to thymine edits in mitochondria. Here, Mok et al. have used similar […]