Entries by Mary Williams

Review: Using CRISPR/Cas in three dimensions: towards synthetic plant genomes, transcriptomes and epigenomes

Puchta reviews the different ways that CRISPR/Cas9 can be used in synthetic biolgy, beyond the common gene-editing function. For example, the Cas9 protein can be fused to other proteins that activate or repress transcription, and targeted to different promoters using guide RNAs. He further explores the possibility of one day producing synthetic plant genomes. Plant […]

Quantitative characterization of genetic parts and circuits for plant synthetic biology

Control of gene expression involves both activation and repression of transcription. Schaumberg et al. used transient expression in Arabidopsis and sorghum protoplasts and dual-luciferase outputs to rapidly quantitate synthetic repressors and repressible promoters, and verified their results in transgenic Arabidopsis plants. Nature Methods 10.1038/nmeth.3659