Ahmed Alabd, first author of “ABRE-BINDING FACTOR3-WRKY DNA-BINDING PROTEIN44 module promotes salinity-induced malate accumulation in pear”
Ph.D. Candidate at College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China
2018-now: Ph.D. student, Zhejiang University, Hangzhou, China;
20??-2016: M.Sc. Alexandria University, Egypt;
20??-2011: B.Sc. Alexandria University, Egypt.
Playing and watching sports, reading and cooking
I’m currently studying at the College of Agriculture and Biotechnology, Zhejiang University, China. Since joining Prof Yuanwen Teng’s lab in 2018, I have been conducting research on the regulatory mechanisms of anthocyanins and malic acid in fruits under the supervision of of Prof Yuanwen Teng, Dr Songling Bai and Dr Junbei Ni. In this research, we revealed a regulatory mechanism by which salinity affects malate accumulation. Genetic and biochemical analyses showed the key roles of PpWRKY44 and ABRE-BINDING FACTOR3 (PpABF3) transcription factors in promoting malate accumulation in response to salinity. We found that PpWRKY44 is involved in salinity-induced malate accumulation by binding directly to the W-box on the promoter of the malate-associated gene PpALMT9 (aluminium-activated malate transporter 9) to activate its expression. A series of in vivo and in vitro assays showed that the G-box cis-element in the promoter of PpWRKY44 was targeted by PpABF3, which further enhanced salinity-induced malate accumulation. This research provides new insights into the regulatory mechanism of malate accumulation in pear fruit in response to salinity.