Yang Yang, co-first author of “Multiple cyclic nucleotide-gated channels function as ABA-activated Ca2+ channels required for ABA-induced stomata closure in Arabidopsis”
Current Position: Ph.D. candidate at CAS Center for Excellence in Molecular Plant Science (CEMPS) and University of Chinese Academy of Sciences (UCAS), Shanghai, China.
Education: Expected to graduate in the Fall of 2023 at CEMPS and UCAS.
Non-scientific Interests: Reading, Watching table tennis match.
Brief bio: I joined Dr. Yong-Fei Wang’s laboratory at CEMPS since 2018 to pursue my Ph.D. My research is mainly focused on how CNGCs regulate ABA-induced stomatal movement as Ca2+ channels and cytosolic Ca2+ signaling regulators. Our findings showed that the quadruple loss-of-function mutant cngc5-1cngc6-2cngc9-1cngc12-1 (cngc5/6/9/12) was strongly ABA-insensitive in stomatal movement and transpirational water loss. Further analysis revealed that ABA-induced cytosolic Ca2+ oscillations were dramatically disrupted, and ABA-activation of inward Ca2+ channel currents of guard cells was significantly inhibited in cngc5/6/9/12 compared with wild type. These results demonstrate that multiple CNGCs, mainly including CNGC5, CNGC6, CNGC9, and CNGC12, are the key components of ABA-activated plasma membrane Ca2+ channels in guard cells for ABA-specific cytosolic Ca2+ signature to regulate ABA-induced stomatal movement and transpirational water loss in Arabidopsis.
发表论文：Multiple cyclic nucleotide-gated channels function as ABA-activated Ca2+ channels required for ABA-induced stomata closure in Arabidopsis
个人简历： 我从2018年加入到中国科学院分子植物科学卓越创新中心王永飞研究员团队来攻读博士学位。我的课题主要是研究CNGC是如何作为质膜Ca2+通道和胞内Ca2+信号调节器来参与调控ABA诱导的气孔关闭。我们的研究结果显示，功能缺失四突变体cngc5-1cngc6-2cngc9-1cngc12-1（cngc5/6/9/12）的气孔关闭和蒸腾失水表现出对ABA强烈不敏感的表型。进一步的研究发现，与野生型相比，ABA激活的cngc5/6/9/12气孔保卫细胞质膜内向Ca2+电流大幅度减小，ABA诱导的cngc5/6/9/12气孔保卫细胞胞质Ca2+振荡模式，包括Ca2+升高幅度和震荡周期，被显著改变。这些结果说明CNGC5, CNGC6, CNGC9和CNGC12作为主要组分，共同参与组成了一类ABA激活的拟南芥气孔保细胞质膜Ca2+通道，并作为ABA信号网络中的关键组分参与调控ABA诱导的气孔关闭运动。