Current Position: Research assistant
Education: Ph.D. in Botany from CAS Center for Excellence in Molecular Plant Sciences (CEMPS) and University of Chinese Academy of Sciences (UCAS).
Non-scientific Interests: Reading, Music
Brief bio: I joined Prof. Yong-Fei Wang’s group at CEMPS since 2011. My research is mainly focused on the characterization of the main Ca2+ channels required for important biological processes, including the regulation of stomatal movements and polar growth of plant cells. I have successfully revealed that three CNGC members (CNGC5, CNGC6 and CNGC9) are involved in polar growth of root hairs by regulating cytosolic Ca2+ gradients in Arabidopsis root hair tips. That work was published in another journal about two years ago. In this research, I generated three dominant negative (DN) transgenic Arabidopsis lines, and observed strong ABA-insensitive phenotypes of stomatal movement and transpirational water loss. These phenotypes observed in the DN lines suggest that CNGC channels are required for ABA regulation of stomatal movement and transpirational water loss. Thus I further generated a number of transgenic Arabidopsis lines by expressing the GUS encoding gene under the promoters of CNGC family members, and revealed the highly expression of five CNGCs in Arabidopsis guard cells, namely CNGC2, CNGC5, CNGC6, CNGC9, and CNGC12. Based on this data, I further generated a CNGC quadruple mutant cngc5-1cngc6-2cngc9-1cngc12-1 (c5/6/9/12), the complemented lines of the quadruple mutant and overexpression lines in wild type background, and observed strong ABA insensitive phenotypes of stomatal movement and transpirational water loss in the quadruple mutant, similar to that of the three DN lines. I and my collaborators further conducted a number of experiments, and eventually revealed that multiple CNGCs, mainly including CNGC5, CNGC6, CNGC9, and CNGC12, constituted a type of ABA-activated Ca2+ channels, which were involved in ABA regulation of stomatal movement and transpiration water loss by mediating external Ca2+ influx and regulating ABA-specific cytosolic Ca2+ signature in guard cells. I believe that our findings opened a door for many other studies to expand our understandings about the underlying molecular mechanisms of upstream stimulus-specific cytosolic Ca2+ signaling in plant cells.
发表论文：Multiple cyclic nucleotide-gated channels function as ABA-activated Ca2+ channels required for ABA-induced stomata closure in Arabidopsis