Yanfei Zhang, first author of “Wheat TaSnRK2.10 phosphorylates TaERD15 and TaENO1 and confers drought tolerance when overexpressed in rice”
Current Position: Ph D candidate in Henan Agricultural University, Zhengzhou, China.
Education: Ph D candidate (Henan Agricultural University, Zhengzhou, China), Master of Agriculture (Henan Agricultural University, Zhengzhou, China), Bachelor of Agriculture (Henan Agricultural University, Zhengzhou, China).
Non-scientific Interests: Playing badminton, travelling, climbing mountain
Brief bio: As a Ph D student of Henan Agricultural University, I have the honor to join Dr. Mao Xinguo’s team at the Institute of Crop Sciences, Chinese Academy of Agricultural Sciences. My researches mainly focus on drought tolerance in wheat. We identified and characterized a key drought response gene TaSnRK2.10 with omics and molecular biological methods. Our data showed that TaSnRK2.10 acts as a positive regulator in the response to drought stress at different levels. TaSnRK2.10 was induced by multiple abiotic stresses and phytohormones. Ectopic expression of TaSnRK2.10 confers enhanced drought tolerance in rice, manifested by multiple improved physiological indices, including increases in water content, cell membrane stability, and survival rates, as well as decreases in water loss and accumulation of H2O2 and malonaldehyde. TaSnRK2.10 interacts with and phosphorylates TaERD15 (Early Responsive to Dehydration 15) and TaENO1 (Enolase 1) in vivo and in vitro. Overexpression of TaERD15 causes sensitivity to drought stress. TaERD15 phosphorylated by TaSnRK2.10 is prone to degradation by the 26S proteasome, thereby mitigating its negative effects on drought tolerance. Ectopic expression of TaSnRK2.10 results in a significant increase of phosphoenolpyruvate (PEP), a key metabolite of primary and secondary metabolism in transgenic plants, thereby enhancing their viability under drought stress. Our results demonstrate that TaSnRK2.10 not only regulates stomatal aperture and the expression of drought-responsive genes, but also enhances PEP supply and promotes the degradation of TaERD15, all of which enhance drought tolerance.
All co-authors and I are sincerely delightful and honored that our work was accepted to be published in Plant Physiology.