Xiufang Hong, first author of “Ascorbate peroxidase 1 allows monitoring of cytosolic accumulation of effector-triggered reactive oxygen species using a luminol -based assay”
Current Position: Ph.D candidate in Plant Pathology, Zhejiang University
Education: Bachelor of Plant Sciences & Technology, Huazhong Agricultural University
Non-scientific Interests: Music and traveling
After graduating from Huazhong Agricultural University with a bachelor’s degree in 2018, I was honored to join the group of Prof. Yan Liang, Zhejiang University. During my PhD studies, I mainly focused on investigating the molecular mechanism underlying the regulation of the long-lasting ROS burst in plant immunity. In this study, we first performed a forward genetic screen and isolated Arabidopsis mutants called delt4 that exhibited abolished sustained ROS production after LPS treatment. Based on the combination of Map-based cloning and whole genome sequencing, we found that the delt4 phenotype was indeed caused by the mutation in APX1 encoding a cytosolic ascorbate peroxidase. Using different ROS detection methods, we found that the peroxidase not only acts as an antioxidant to regulate intracellular redox homeostasis, but also catalyzes luminol oxidation and allows monitoring of the kinetics of cytosolic long-lasting ROS. Furthermore, we revealed that the sustained ROS induced by the effector of avirulent bacteria are eventually accumulated in the cytosol with the use of fluorescent dyes. This study sheds light on the novel function of cytosolic ascorbate peroxidase and also provides important insights into the spatial dynamics of ROS accumulation in plant immunity.