Recent Posts

A chloroplast protein atlas reveals punctate structures and spatial organization of biosynthetic pathways

Chloroplasts are the location of key processes including photosynthesis, starch synthesis and lipid synthesis. However, many chloroplast proteins have unknown functions,  a problem that can in part by addressed through high-resolution localization data. Here, 1034 putative chloroplast- localized proteins…

Recruiting the ATP-generating nanomotor in chloroplasts

Reiter et al. investigate the molecular basis for coupling factor CF1 recruitment in Arabidopsis thaliana. The Plant Cell (2022). By Thilo Rühle Background: Thylakoid ATP synthases are impressive molecular engines that harness the light-driven proton gradient to generate ATP during photosynthesis.…

Discovering autophagy protein cargo by protein turnover analysis in Arabidopsis

Li et al. explore the nature of proteins that accumulate in mutants deficient in autophagy machinery. Plant Cell Lei Li1,2 and A. Harvey Millar2 Frontiers Science Center for Cell Responses, Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, 300071 Tianjin,…

TOC-TIC supercomplex structure

In an evolutionary plot twist, most of the proteins needed inside the chloroplast are encoded in the nucleus and translated in the cytosol as preproteins. The preproteins are imported into the chloroplast through two membranes (outer and inner). Genetic and biochemical approaches have revealed many of…

Orf2971: a link between chloroplast import and quality control in Chlamydomonas

Xing et al. investigate the link between chloroplast import and quality control in Chlamydomonas reinhardtii. Plant Cell https://doi.org/10.1093/plcell/koac180 By Jiale Xing, Junting Pan and Wenqiang Yang Background: The chloroplast is an important bioreactor as well as a photosynthetic site. Approximately…

Chloroplasts: reporting at the door

Loudya et al. report that complementary mutations in TIC100 of the chloroplast inner envelope membrane can cause reductions or corrective improvements in chloroplast protein import, highlighting a role in signalling. Plant Cell https://doi.org/10.1093/plcell/koac153 By Naresh Loudya, Paul Jarvis and…

The chloroplast protein biogenesis network at the ribosome during heat acclimation

By Raphael Tröscha, Fabian Riesb, Lisa Désirée Westrichb, Felix Willmundb, a Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany; b Molecular Genetics of Eukaryotes, University of Kaiserslautern, Paul-Ehrlich-Str. 23, 67663 Kaiserslautern, Germany Trösch,…

Chloroplast protein import determines plant proteostasis and retrograde signaling (bioRxiv)

The neurological condition Huntington’s disease is caused by mutated versions of the Huntingtin gene that encode expanded stretches of polyglutamine repeats, resulting in protein aggregation. However, despite the widespread presence of polyglutamine repeat regions in plant proteomes, similar pathologies…

A synthetic switch based on orange carotenoid protein to control blue-green light responses in chloroplasts (Plant Physiol)

Synthetic biology aims to engineer and redesign components of natural organisms for useful purposes.  One of the most prolific areas of synthetic biology is based on the engineering of photoreactive proteins with signaling potential, such as photoreceptors. Natural photoreceptors consist of a prosthetic…