Tag: Biochemistry

As photosynthetic autotrophs, plants have the potential to convert sunlight into a vast array of useful products: to act as little green metabolic factories. Of course, they already provide us with everything from carbohydrates and vitamins to stimulants and medicinal compounds, but with a few small…

Activity-based protein profiling is a proteomics approach that involves covalently labeling reporter tags to subsets of proteins based on their active sites. Morimoto and van der Hoorn define different types of probes and the types of proteins that they bind to. Activity profiling can reveal different…

Some bees are generalist pollinators that gather pollen from a wide range of species, whereas others are specialists that visit only one or a few species. Vanderplanck et al. examined floral traits of the host plants of two different groups of generalist bees. There was no significant correlation between…

Fusarium oxysporum is a fungal pathogen of plants. F. oxysporum f. sp. conglutinans (Foc) causes fungal wilt in cabbage. Two races have been identified, with Race 2 being much more pathogenic than Race 1. Li et al. used a proteomic approach to investigate the origin of Race 2’s enhanced pathogenicity.…

PATHOGENESIS-RELATED 1 (PR-1) protein was identified 50 years ago as a small protein induced in response to pathogens, but its mode of action has remained obscure.  PR-1 is a member of the CAP family (cysteine-rich secretory protein, antigen 5, and pathogenesis-related 1). These proteins share a 150…

Structural biology provides a key link between genotype and phenotype. Hirano et al. probe the structure of a heterodimer of plant-specific GRAS family transcriptional regulators [SHORT-ROOT (SHR) and SCARECROW (SCR)] as bound to the transcription factor JACKDAW (JKD). The GRAS family proteins (encoded…

A considerable knowledge gap remains between the plant genome and the plant metabolome. To address this, Schläpfer et al. have developed a computational pipeline to identify metabolic enzymes, pathways, and gene clusters. Although metabolic genes are known to cluster in bacteria and fungi, until recently…

The distribution of metabolites in tissues can be determined in situ through the technique Mass spectrometry imaging (MSI) with matrix-assisted laser desorption ionization (MALDI) (also known as MALDI-MSI). Duenas et al. used MALDI-MSI to analyze the distribution of thylakoid membrane lipids in maize,…