Shuai Wang: The Plant Cell First Author

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Shuai Wang, co-first author of “Phosphorylation and ubiquitination of OsWRKY31 are integral to OsMKK10-2-mediated defense responses in rice”

Current Position: Ph.D. at College of Plant Protection, China Agricultural University，China

Education: M.A. at College of plant Science and Technology, Beijing University of Agricultural，China

Non-scientific Interests: Sports and Reading

Brief bio: The undergraduate and master level preliminary contact with molecular biology experiments, which gave me a strong interest in the molecular mechanism of plant disease resistance regulation. In 2017, I had the honor to be a doctoral student of Professor Guo Zejian, and began to engage in the research of rice WRKY transcription factors in regulating disease resistance. Our group identified OsWRKY31, a positive regulator of resistance to M. oryzae and found that OsWRKY31, OsMPK3 and OsMKK10-2 interact in the nucleus and function in a complex. Activation of OsMKK10-2 is able to increase the accumulation of jasmonic acid and salicylic acid, thereby enhancing M. oryzae resistance and inhibiting plant growth by reducing the level of indole-3-acetic acid. Whereas, as a downstream transcription factor of OsMKK10-2, knockout of OsWRKY31 impairs OsMKK10-2-mediated defense responses. Phosphorylated form of OsWRKY31 not only enhanced protein stability but also had high DNA binding activity and enhanced resistance to M. oryzae. Moreover, OsWRKY31 also undergo ubiquitination through the E3 ubiquitin ligase OsREIW1 to regulate the self- stability. Currently, I am studying for a PhD and my current research is to further explore the interactive response of OsWRKY31 in phosphorylation and ubiquitination in disease resistance, in order to gain a deeper understanding of the precise regulation of defense and growth balance.

姓名：王帅

目前职位：中国农业大学，博士研究生

教育经历：北京农学院，硕士

兴趣爱好：运动，阅读

个人简介：本科和硕士阶段初步接触分子生物学实验，使我对植物抗病调控的分子机理产生了浓厚的兴趣。2017年，我有幸成为郭泽建教授的博士研究生，开始从事水稻WRKY转录因子参与调控抗病性相关研究。我们课题组发现了一个对水稻稻瘟病菌抗性的正调控因子OsWRKY31，研究发现OsWRKY31、OsMPK3和OsMKK10-2以复合体形式互作于细胞核并发挥功能。OsMKK10-2的激活能够增加茉莉酸和水杨酸的积累，从而增强水稻稻瘟病菌抗性，并且通过降低吲哚-3-乙酸水平来抑制植株生长。而作为OsMKK10-2的下游效应因子，敲除OsWRKY31会损害OsMKK10-2介导的防御反应。拟磷酸化形式的OsWRKY31不仅增强了蛋白稳定性，还具有较高的DNA结合活性，并增强了对稻瘟病菌的抗性。此外， OsWRKY31还通过E3泛素连接酶OsREIW1发生泛素化作用以调控自身蛋白稳定性。目前，我正在攻读博士学位，我当前的研究是进一步探究OsWRKY31参与的磷酸化修饰与泛素化修饰在抗病过程中的交互应答，以期在精细调控防御和生长平衡方面取得更深入的了解。