Shiquan Bian: Plant Physiology First Author

Shiquan Bian, first author of “NtMYB305a Binds to the Jasmonate-Responsive GAG Region of NtPMT1a Promoter to Regulate Nicotine Biosynthesis”

Current Position: Ph.D. Candidate, Chinese Academy of Agricultural Sciences

Education: Bachelor, Qingdao Agricultural University; Master, Yangzhou University

Non-scientific Interests:  Reading, Music

Brief bio:

I joined Professor Hongbo Zhang’s research team at the Functional Component Research Center of Tobacco Research Institute, Chinese Academy of Agricultural Sciences and began my PhD study in 2018. During this period, my main research task is to study the regulation of nicotine biosynthesis in tobacco. We found that R2R3-MYB transcription factor NtMYB305a specifically recognizes and binds to the AT-rich motif of the jasmonate-responsive GAG region within the promoter of NtPMT1a, encoding putrescine N-methyltransferase that catalyzes the first-committed step of nicotine biosynthesis. Functional studies revealed that NtMYB305a positively regulates nicotine biosynthesis in tobacco, and cooperates with bHLH transcription factor NtMYC2a to regulate this process. This work sheds novel insights into the molecular regulation of nicotine biosynthesis.

 

姓名：卞士权

职位：中国农业科学院烟草研究所，在读博士研究生

学历：青岛农业大学，学士学位；扬州大学，硕士学位

兴趣爱好：阅读，音乐

个人简介：我于2018年进入中国农业科学院烟草研究所功能成分研究中心张洪博研究员课题组攻读博士学位。烟草腐胺甲基转移酶在尼古丁合成过程中发挥关键作用，我在攻读博士学位期间针对腐胺甲基转移酶基因NtPMT1a的转录调控进行了尼古丁的生物合成调控研究。我们发现R2R3-MYB转录因子NtMYB305a可以特异识别并结合NtPMT1a启动子的茉莉酸应答区段中的富AT元件。基因功能研究证明NtMYB305a可以正向调控烟草的尼古丁合成，并与bHLH转录因子NtMYC2a协同调控这一生物过程。本研究加深了我们对烟草尼古丁代谢调控分子机制的了解。