Recognizing The Plant Cell authors: Xiaoman Yang

Xiaoman Yang, co-first author of Cytochrome b5 is an obligate electron shuttle protein for syringyl lignin biosynthesis in Arabidopsis

Current Position: PhD student at South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong. Also worked as a visiting student for 2 years in Chang-Jun Liu’s lab in Brookhaven National Laboratory, Upton, NY.

Education: PhD: 2013-present, South China Botanical Garden, Chinese Academy of Sciences; Bachelor: 2009-2013, South China University of Technology

Non-scientific Interests: Swimming, Badminton

Brief Bio: Currently, I’m a PhD candidate in Biochemistry and Molecular Biology as a major field of study. I have completed a bachelor degree in Food Quality and Safety. Though a substantial change in my field of study thereafter, it really made me feel satisfied and curious to explore further horizons of plant science at the molecular level.

During the master study, my research work was based on prenyltransferase from Fusarium oxysporum. And my PhD research project is concerned mainly on anthocyanin biosynthesis, especially glycosylation in the fruit of Lycium ruthenicum Murr. (Black Goji berry), a traditional Chinese medicinal plant.

From 2016 to 2018, I was a visiting student in Chang-Jun Liu’s lab at Brookhaven National Laboratory. The research experience really broadened my vision regarding compound synthesis in plants. I had jointed in a project related to elucidate the protein complex involved in three main P450 enzymes in lignin biosynthesise in Arabidopsis thaliana. We found that a cytochrome b5 D isomer (CB5D), interacted with C3’H, C4H and F5H in Arabidopsis, but only affected S-lignin content. I conducted in vitro assay and showed that disrupting the gene for CB5D primarily impairs F5H activity. We concluded that cytochrome b5 is an obligate electron shuttle protein for syringyl lignin biosynthesis in Arabidopsis based on genetic and biochemical evidence.

 

姓名:杨小满

职位:博士生,中国科学院华南植物园,2016年国家留学基金委项目在美国Brookhaven National Laboratory交流2年

兴趣爱好:游泳,羽毛球

科研经历简介:从本科的食品质量与安全专业转到了研究生的生物化学与分子生物学,微观的世界让我感觉更加有新鲜感和成就感。硕士期间主要进行香蕉镰刀菌异戊烯基转移酶的研究。博士课题则主要在药用植物领域,黑果枸杞是我主要的研究材料,我们的研究目的是试图解释黑果枸杞花青苷合成途径中的糖基化修饰。期间,有幸加入美国Brookhaven National Laboratory的Chang-Jun Liu实验室,Liu lab集中以杨树和拟南芥作为研究对象,主要研究植物木质素的生物合成及相关调控机理。在这个实验室的学习经历又进一步开阔了我在植物研究领域的眼界。访问期间,我主要参与了模式植物拟南芥木质素合成途径中3个重要的P450蛋白(C3’H, C4H, F5H)及与其相互作用的膜蛋白的研究,膜蛋白之间的互作以不同的机制对植物中木质素造成不同的影响。在本研究中我们发现F5H与细胞色素b5的D异构体(CB5D)相互作用能够响拟南芥中