Recognizing Plant Cell authors: Feifei Xu

Feifei Xu, first author of Exportin-4 Coordinates Nuclear Shuttling of TOPLESS Family Transcription Corepressors to Regulate Plant Immunity

Current Position: Research associate at the Institute of Nuclear Agricultural Sciences, Zhejiang University, Zijingang Campus, Hangzhou, China

Education: PhD in Biophysics, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China

Non-scientific Interests: reading and painting

Brief bio: I joined Dr. Yangnan Gu’s lab as a postdoctoral scholar in 2017 at Tsinghua University in China. I focused on characterizing the role of nuclear transport receptors (NTRs) in plant innate signaling. Through a CRISPR/Cas9-based targeted genetic screen, I found that Exportin 4 (XPO4) in Arabidopsis confers strong genetic interaction with CPR5, a key regulator of effector triggered immunity (ETI) located at the nuclear pore complex. By leveraging the newly developed proximity-labeling proteomics, I profiled XPO4 substrates and identified TOPLESS family transcription corepressors as XPO4-specific cargo. We propose that XPO4 coordinates the nuclear accumulation of TPL/TPRs, which plays a role in regulating SA-mediated defense feedback to modulate immune strength downstream of CPR5 during ETI induction. My current research involves in identifying new components of the rice starch biosynthetic enzyme complex and studying their functions in regulating eating and cooking qualities of rice grain.




教育背景:浙江大学,生物物理学 博士


个人研究简介:2017年加入清华大学生命学院谷杨楠研究员课题组从事博士后研究,主要研究方向为核转运受体在植物免疫调控中的作用。我们之前发现CPR5蛋白是定位于核孔复合体上的重要免疫调控因子。 通过在cpr5自免疫突变体背景中对核转运受体家族基因进行CRISPR/Cas9基因编辑筛选,发现xpo4缺失突变体为cpr5的特异免疫增强子,双突变体呈现致死性免疫激活。利用TurboID技术联合定量质谱分析,我们获得了XPO4的转运底物图谱,并发现XPO4特异性将TOPLESS家族转录抑制因子运输出细胞核。此过程对负反馈调节由水杨酸介导的cpr5自免疫信号扩增中起到关键作用。目前我在浙江大学原子核农业科学研究所工作,主要研究方向为水稻淀粉合成酶复合体新组分的挖掘及其在调控稻米蒸煮与食用品质中的作用。