Jiaqi Tang: The Plant Cell First Author

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Jiaqi Tang, co-first author of “WRKY53 negatively regulates rice cold tolerance at the booting stage by fine-tuning anther gibberellin levels”

Current Position: Research associate, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China

Education: Ph.D., Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China

Non-scientific Interests: walking, painting, reading

Brief bio:

In 2016, I joined Bu Qingyun’s lab in the Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, and started my Ph.D study. My main work is to explore the genes regulating cold tolerance at booting stage in rice and analyze the mechanism. Our study found that WRKY53 negatively regulates cold tolerance of rice at booting stage. WRKY53 directly bind to the promoters of GA biosynthesis genes and transcriptionally represses their expression in anthers, and genetic evidence supports that WRKY53 acts genetically upstream of GA biosynthesis. In addition, we also found a novel mechanism of GA regulating rice fertility that SLR1, which is a key negative regulator of GA signaling, that can interact with two critical transcription factors for tapetum development, UDT1 and TDR, and GA derepresses the sequestration of SLR1 on the transcription activity of UDT1 and TDR. More importantly, knocking out WRKY53 in diverse varieties increases cold tolerance without a yield penalty, leading to a higher yield in rice subjected to cold stress.

In this study, we identified a novel cold tolerance regulation gene WRKY53 at booting stage, which provides important production value for molecular breeding of cold tolerance at booting stage in rice.

 

姓名：唐佳琦

发表论文：WRKY53 negatively regulates rice cold tolerance at the booting stage by fine-tuning anther gibberellin levels

目前职位：中国科学院东北地理与农业生态研究所，特别研究助理

教育背景：中国科学院大学，理学博士

爱好：徒步，绘画，阅读

科研经历简介：

我于2016年加入到中国科学院东北地理与农业生态研究所卜庆云老师课题组开始硕博连读学习生活。我的研究方向是挖掘水稻孕穗期耐冷调控基因并对其进行机制解析。我们的研究发现WRKY53能够负向调控水稻孕穗期耐冷性；深入研究发现WRKY53可以直接结合GA生物合成基因GA20ox1、GA20ox3、GA3ox1，并对其起到转录抑制作用，遗传学实验也进一步证明了WRKY53在调控水稻孕穗期耐冷性上位于GA生物合成基因GA20ox1、GA20ox3、GA3ox1的上游。此外，我们还发现GA信号关键负调控因子SLR1可以与绒毡层发育关键基因UDT1、TDR互作，通过影响UDT1、TDR的DNA结合能力及转录激活活性来参与调控水稻育性。更重要的是，在不同背景下wrky53突变体均能显著提高孕穗期耐冷性，且不影响正常条件下的水稻产量，但孕穗期低温胁迫对wrky53水稻材料的减产显著降低。

本研究发现了一个新的孕穗期耐冷调控基因WRKY53，对水稻孕穗期耐冷分子育种提供了重要的生产价值。