Xiaojie Tian, co-first author of “WRKY53 negatively regulates rice cold tolerance at the booting stage by fine-tuning anther gibberellin levels”
Current Position: Associate Research Fellow, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China
Education: Ph.D., Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China
Non-scientific Interests: riding, swimming, dancing
In September 2012, I joined the professor Bu’ lab in the Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, and started my Ph.D study. We found that the transcription factor OsWRKY53 is a positive regulator of BR signaling. OsWRKY53 as the common phosphorylated substrate of OsGSK2 and OsMAPK6. OsGSK2 reduces its protein stability by phosphorylating OsWRKY53, while OsMAPK6 improves its biological function by phosphorylating OsWRKY53. OsWRKY53 integrates classic brassinosteroid signaling and the mitogen-activated protein kinase pathway to regulate rice architecture and seed size. Relevant works were published in The Plant Cell and Plant Physiology, respectively. In addition, in the rice cold tolerance at booting stage, we found that the cold tolerance of oswrky53 mutants at booting stage are significantly improved and with higher contents of gibberellin (GA) in anthers. OsWRKY53 fine-tuning GA levels by repressing the expression of GA biosynthesis genes OsGA20ox1, OsGA20ox3, and OsGA3ox1. It is clarified that the new function and mechanism of OsWRKY53 in negatively regulates rice cold tolerance at the booting stage.
本人于2012年9月进入中科院东北地理与农业生态研究所卜庆云研究员团队进行硕博学习。发现水稻转录因子OsWRKY53是BR信号的一个正调控因子。OsWRKY53分别是OsGSK2和OsMAPK6的磷酸化底物，OsGSK2通过磷酸化OsWRKY53降低其蛋白稳定性，而OsMAPK6通过磷酸化OsWRKY53提高其生物功能。OsWRKY53介导了BR和MAPK信号间的交叉对话，正向调控水稻的株型和粒型。相关工作分别发表于The Plant Cell和Plant Physiology。此外，在孕穗期耐冷方向上，发现oswrky53突变体孕穗期耐冷性显著提高，花药GA含量显著增多；OsWRKY53通过抑制GA生物合成基因GA20ox1、GA20ox3、GA3ox1的表达，调控花药GA含量，解析了OsWRKY53负向调控水稻孕穗期耐冷性的新功能和新机制。