Entries by Mary Williams

Designer pentatricopeptide repeat PPR protein as RNA affinity tag (Plant Cell)

Pentatricopeptide repeat (PPR) proteins bind RNA, using a code in which specific amino acid motifs recognize specific bases, similar to TAL effector proteins. In plants, PPR proteins localize almost exclusively to mitochondria and chloroplasts, with functions including RNA, stabilization, splicing and editing. Here, McDermott et al. used insights about the amino-acid to RNA code to […]

Review: Source–sink regulation in crops under water deficit ($) (Trends Plant Sci)

Plants have a remarkable ability to coordinate cellular activities across huge distances, yet we have only a basic understanding of how these remote activities are coordinated. A review by Rodrigues et al. summarizes what we know about the relationship between source (e.g., photosynthetic tissues) and sinks (e.g., growing tissues or seeds), and particularly how this […]

Review: Integration of sulfate assimilation with C and N metabolism in transition from C3 to C4 photosynthesis (J Exp Bot)

“Cysteine (HO2CCH(NH2)CH2SH) synthesis is the converging point of the three major pathways of primary metabolism: carbon, nitrate, and sulfate assimilation.” It’s hard enough to coordinate two pathways, let alone three; but these metabolic connections are revealed in that a deficiency in one nutrient affects assimilation rates of the others. As yet, the connections between pathways […]

Multi-level modulation of light signaling by GIGANTEA regulates both the output and pace of the circadian clock ($) (Devel Cell)

The daily cycle of light and dark controls many facets of plant growth and development, but these cycles depend on and are most efficient when integrated with the rhythms of the circadian clock. Nohales et al. have identified a key mechanism that directly links the regulation of light-responsive genes and the regulation of the clock. […]

Three-dimensional time-lapse analysis of maize root system archictecture (Plant Cell)

Root system architecture (RSA) profoundly affects plant nutritent uptake and response to drought, and is also famously extremely developmental plastic, which makes it difficult to identify genes that control root growth traits. Here, Jiang et al. analyzed 3D growth patterns over time of three maize lines (Mo17, B73 and their hybrid) growing in a gel-based […]

A diversity of traits contributes to salinity tolerance of wild Galapagos tomatoes seedlings (bioRxiv)

Domestication has been accompanied by a decrease in genetic diversity, so efforts to improve stress tolerance can be aided by exploring the crop’s wild relatives. Here, Pailles et al. examined salt tolerance in Galapagos tomatoes (Solanum cheesmaniae and Solanum galapagense), which grow “constantly splashed with seawater”. After growing the plants in 200 mM NaCl, the […]

Plant Science Research Weekly: May 31st

Review: Integration of sulfate assimilation with C and N metabolism in transition from C3 to C4 photosynthesis “Cysteine (HO2CCH(NH2)CH2SH) synthesis is the converging point of the three major pathways of primary metabolism: carbon, nitrate, and sulfate assimilation.” These metabolic connections are revealed in that a deficiency in one nutrient affects assimilation rates of the others. […]

Default activation and nuclear translocation of the plant cellular energy sensor SnRK1 ($) (Plant Cell)

When I’m using my laptop without it being plugged in, at some point it enters battery saving mode as it senses that the battery charge is getting low. Organisms also sense their low energy reserves and activate alternate metabolic processes to ensure survival. The core energy sensor is a hetero-trimeric complex conserved in eukaryotes (yeast, […]

Drop-Seq for plants: High-throughput, single cell transcriptomics (Cell Reports)

Drop-Seq is an exciting high-throughput technology for single cell transcriptomics. Using microfluidics, single cells are encapsulated in drops with beads carrying “bar-coded” primers. The RNA from each individual cell is labeled with a unique primer code, then sequenced. Now, Shulse et al. have adapted this method for use with plant cells (requiring an additional protoplasting […]