Entries by Mary Williams

Phosphatidylinositol 3-phosphate–binding protein AtPH1 controls the localization of the metal transporter NRAMP1

NRAMPs are transporters of iron (Fe) and manganese (Mn). The nramp3nramp4 double mutant arrests shortly after germination, due to its inability to remobilize Fe from seed vacuolar stores. Agorio et al. used a genetic approach to identify nns1, a partial suppressor of the growth-arrest phenotype. They mapped nns1 to AtPH1, a gene encoding a pleckstrin-homology […]

Light sheet fluorescence microscopy quantifies calcium oscillations in root hairs

Light sheet fluorescence microscopy (LSFM) is a powerful imaging tool; unlike confocal microscopy, which illuminates a sample one small volume at a time, LSFM illuminates a complete optical section at a time, greatly speeding up the imaging process and lowering phototoxic effects. Candeo et al. use close-to-physiological imaging of tip-growing root hairs through the combination […]

Intron DNA sequences can be more important than the proximal promoter in determining the site of transcript initiation

The site at which transcription begins is traditionally thought to be determined in the proximal promoter by assembly of the pre-initiation complex just upstream of the transcription start site (TSS). New results from Gallegos and Rose challenge this assumption. Prior studies have shown that the presence of an intron near the TSS can boost transcript […]

Cell-free membrane protein expression system enables functional characterization of receptor-like protein kinase FERONIA ($)

Membrane proteins are some of the most interesting cellular proteins, serving as sensors and transducers of diverse signals, yet they also are the most challenging to investigate because they require lipid interactions for proper structure and function. Recently, cell-free expression systems for membrane proteins have been developed that incorporate lipid nanodiscs consisting of membrane scaffold […]

Update: Ion transport at the vacuole during stomatal movement

Gas exchange and transpiration are regulated by the stomatal aperture, which is itself regulated by the changes in volume of the guard cells that overlay the stomatal pore. When triggered to open, solutes such as K+ and Cl– enter the guard cell through ion transporters, followed osmotically by water; as the cells enlarge, the gap […]

Could plants be sentient?

Sentience, the capacity to feel subjectively, is considered limited to organisms that have a nervous system and a centralized brain. Plants, therefore, have been excluded from this group based on: lack of a transmission mechanism like the animal nervous system; lack of a brain; simplicity; and inability to run away from a threat or to […]