The histone H3 variant H3.3 is distinguished by its expression throughout the cell cycle, while H3.1 is expressed predominantly during DNA replication. Genome-wide ChIP studies in plants have shown that H3.3 is associated with actively transcribed genes, and enriched near the transcriptional end sites. This pattern also overlaps with the enrichment of RNA Polymerase II. […]
Zhang et al. showed that POSITIVE REGULATOR OF IRON HOMEOSTASIS 1 (OsPRI1), a bHLH transcription factor, is an interacting partner of the iron-binding sensor OsHRZ1. A loss-of-function mutation of OsPRI1 is responsible for a hypersensitive response to Fe deficiency. OsPRI1 works downstream of OsHRZ1 and directly controls the expression of OsIRO2 and OsIRO3 by binding […]
Cadmium is a toxic metal in our environment. Bruno et al. explored cadmium-mediated root-growth inhibition using the model plant Arabidopsis thaliana. Cadmium affects root growth longitudinally by reducing root meristem cell number and radially by controlling the number and width of stele cells. The authors showed that misexpression of the trasncription factor SCARECROW (SCR) occurs […]
Fungal invasion exploits plant cell membrane trafficking components. For instance, plant immunity against fungi such as Vlumeria graminis f.sp. hordei (Bgh) is defined through two stages: pre- and post-invasive immunity. Nielsen et al. found that Vacuolar Protein Sorting 9a (VPS9a) acts as a guanine nucleotide exchange factor which activates the Rab5 GTPase family (ARA6, ARA7 […]
ARMADILLO-REPEAT KINESIN 1 (ARK1) promotes microtubule disassembly and NIMA-RELATED KINASE 6 (NEK6) organizes microtubule arrays. Previous studies showed that NEK6 interacts with ARK1 through Armadillo-repeat (ARM) cargo domain. Eng et al. looked at two constructs: one which lacks ARM (ARK1▲ARM-GFP) and another one with only the ARM domain (ARM-GFP). Interestingly, the authors have found that […]
Chickpea is a highly cultivated member of the legume family, mostly in India, Australia, Pakistan, Turkey, Burma, Iran, Canada and the US. The detrimental threat for chickpea is the invasion of Ascochyta blight, which is caused by the fungal pathogen Ascochyta rabiei (formerly known as Phoma rabiei). The two possible ways to combat this problem are – finding resistance genes […]
Nuclear accidents in recent years such as the Fukushima incident during the tsunami in 2011 revealed the detrimental effects of leaked radioactive cesium (Cs) in environmental soil and water. Due to Cs’s chemical similarity with potassium, an essential macronutrient for plants, cesium is taken up by potassium transporters even when it is present in small […]
DNA sequencing was slow before the development of high throughput sequencing. Portable DNA sequencing, which would make sequencing on-site a reality, was impossible until recently. Parker et al. report on the on-site use of MinION from Oxford Nanopore Technologies for DNA barcoding, which yields data within hours, showing “species identification could be conducted using genome […]
This week’s issue of What We’re Reading is guest edited by Arif Ashraf, a PhD student at Iwate University, Japan, and Graduate Student Ambassador of ASPB. His research interest is understanding the hormonal interplay in primary root development of Arabidopsis thaliana. He blogs about plant science at http://www.aribidopsis.com/. Arif is available in twitter (@aribidopsis) and […]