Entries by Mary Williams

Architecture and dynamics of the jasmonic acid gene regulatory network

Jasmonic acid (JA) and its derivatives including methyl jasmonate (MeJA) mediate diverse responses to wounding and herbivory and also help to control the growth-defense trade off. JA’s core signaling components have mainly been identified through genetics approaches in Arabidopsis, but gaps remain between JA synthesis / receptor interactions and downstream responses.  Hickman and Van Verk […]

MicroRNAs from the parasitic plant Cuscuta campestris target host messenger RNAs

There’s only one thing worse than having a parasite suck the life juices out of you, and that’s having a parasite suck the life juices out of you while simultaneously injecting you with miRNAs to increase your vulnerability. Shahid et al. looked at microRNAs isolated from dodder (Cuscuta campestris), which parasistizes a wide range of […]

Detection of nucleic acid–protein interactions in leaves using fluorescence lifetime imaging microscopy ($)

FRET-FLIM (Fluorescence Resonance Energy Transfer – Fluorescent Lifetime Imaging Microscopy) is a method that allows detection of interactions between two fluorescently-tagged molecules. Camborde and Jauneau et al. describe the use of FRET-FLIM to identify interactions in leaves between GFP-tagged DNA-binding proteins and nucleic acids (DNA or RNA), labeled with Sytox Orange. When the two molecules […]

What We’re Reading: September 8th

Review: Promiscuity, impersonation and accommodation: Evolution of plant specialized metabolism ($) The huge set of chemical pathways beyond the conserved primary metabolic network is described as specialized metabolism (formerly known as secondary metabolism). The diversity of specialized metabolites is due to recent evolutionary innovations in enzyme function, as reviewed by Leong and Last. Key processes […]

Review: Plant signaling and metabolic pathways enabling arbuscular mycorrhizal symbiosis ($)

The relationship between plants and arbuscular mycorrhizal fungi has persisted for 400 million years, but we are still learning about the biochemistry of this interaction. MacLean, Bravo and Harrison review how plants attract, recognize, and accommodate their fungal partners, from pre-contact through nutrient exchange. Recent insights including the role of the NOPE1 N-acetylglucosamine transporter in […]

SPF45-related splicing factor for phytochrome signaling promotes photomorphogenesis by regulating pre-mRNA splicing in Arabidopsis ($)

Light is one of the environmental conditions which regulates plants’ development, but knowledge of how light-induced transcript accumulation occurs via transcriptional versus post-transcriptional mechanisms is limited. Xin et al. report Splicing Factor for Phytochrome Signaling (SFPS) as an interacting partner of the photoreceptor Phytochrome B. The sfps mutant has a hyposensitive response to light and […]

ePlant: Visualizing and exploring multiple levels of data for hypothesis generation ($)

The application of systems biology is quite phenomenal these days for prediction-based modeling and interactive data visualization. Along with the genome sequencing of the model plant Arabidopsis thaliana, there has been a parallel increase in systems biology tools. Unfortunately, these tools have been developed by different groups or institutions for various purposes, whereas a unifying […]