Entries by Humberto Herrera-Ubaldo

AuxSen: a biosensor for direct visualization of auxin (bioRxiv)

Auxins participate in nearly every aspect of plants’ life cycle, but the information about the actual distribution of this hormone is scarce. The location of the hormone has been inferred using reporters based on auxin-transcriptional responses or auxin-dependent degradation signals. For a direct visualization, Herud-Sikimic and colleagues developed AuxSen: a genetically encoded, reversible biosensor for in […]

A practical guide for fluorophore selection for FRET experiments in plants (Plant Direct)

Protein-protein interactions modulate the activities of many proteins, i.e., the signaling specificity of Receptor-like kinases (RLK). Förster Resonance Energy Transfer (FRET) allows the study of the dynamics of protein-protein interactions in live plants; this technique is based on energy transfer from a fluorescent donor to an acceptor fluorophore. There are many fluorophore pairs available for FRET […]

Review: Design and engineering of advanced plant optogenetics systems (TIPS)

Review: Design and engineering of advanced plant optogenetics systems Optogenetics uses light-sensitive proteins to trigger specific outputs in the cell in response to particular wavelengths of light. Many optogenetic systems use chimeric proteins that contain different sensor and effector domains; with this, it is possible to use light to modulate DNA-binding activity, gene expression, or […]

Review: An evolutionary history of genes controlling carpel development (COPB)

Carpels, the female reproductive structures in angiosperms, are the most complex organs in plants. Most of the current knowledge about the molecular mechanism underlying carpel development derives from Arabidopsis. In a new review, Becker summarizes recent studies about the reconstruction of ancestral carpels. Through phylogeny analysis, the lineages of genes and modules within the Arabidopsis […]

Optimal levels of PLETHORA2 for root regeneration capacity (Cell Reports)

Plants can regenerate organs after damage, or even regenerate a whole plant. The regeneration efficiency is different across the organism. In this study, Durgaprasad et al. studied the factors that determine regeneration competence across Arabidopsis roots. They focussed on PLETHORA2 (PLT2), a gene involved in root stem cell maintenance that is expressed in a gradient […]

Carbon nanotube-mediated DNA delivery in intact plants (Nature Protocols)

One promising use of nanomaterials is the delivery of biomolecules into cells. Demirer et al. report a detailed protocol for Carbon Nano Tube-mediated DNA delivery into intact plants, allowing transformation without transgene integration, that can be used in both model and crop species. In brief, carboxylated Single Wall Nano Tubes (SWNT) are modified with a […]

teosinte branched1 and the control of bud dormancy and growth in maize (Nature Comms)

Branching has a significant impact on plant architecture. Maize, a domesticated species, has lower branching levels in comparison to its wild ancestor teosinte. Axillary branching is the result of lateral buds growth. Some factors such as phytohormones and transport of sugars are related to bud activation/dormancy and hence branching. In a recent paper, Dong et […]

Coordination of circadian times in Arabidopsis seedlings (PLOS Biol)

Precise temporal control is crucial for plant developmental processes, e.g., photosynthesis, leaf movement, and flowering. In this work, Greenwood et al. studied how rhythms are coordinated at the whole-organism level. The authors monitored the activity of GIGANTEA (GI), a core clock gene, using a LUCIFERASE reporter gene in Arabidopsis seedlings. GI:LUC expression showed different peaks […]

Review: Developmental thermal responses in Arabidopsis (Plant Physiol.)

Plants can modify their growth in response to environmental challenges. In a recent review, Vu et al. summarize the molecular mechanisms underlying high temperature responses during the Arabidopsis life cycle. At early stages, high temperature promotes abscisic acid (ABA) biosynthesis and decreases gibberellic acid biosynthesis, resulting in germination inhibition. The effect of high ambient temperature […]