
De novo assembly of a new Solanum pennellii accession using nanopore sequencing
Blog, Plant Science Research Weekly, Research, Research BlogChromosomes are long, and DNA sequencing reads have typically been short, meaning that it is necessary to assemble lots and lots of short reads by looking for overlapping sequences. This strategy is made more difficult in repeat-rich and transposon-rich regions of genomes, which characterize many plant…

High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell
Blog, Plant Science Research Weekly, Research, Research BlogThe current version of the Arabidopsis thaliana Col-0 reference genome, TAIR10, still has some gaps and mis-assemblies due to centromeres and repeat-rich regions. In another demonstration of the promise of single-molecule sequencing, Michael, Jupe et al. used a single nanopore flow cell to sequence the…

Genome of wild olive and the evolution of oil biosynthesis
Blog, Plant Science Research Weekly, Research, Research BlogOlive oil is a staple of the healthy “Mediterranean diet” and contains high levels of the monounsaturated fat oleic acid. Unver, Wu et al. present the genome of the wild olive tree (Olea europaea var. sylvestris) (draft sequences of domesticated olive trees without extensive functional annotation…

The Cys-Arg/N-end rule pathway is a general sensor of abiotic stress in flowering plants
Blog, Plant Science Research Weekly, Research, Research BlogThe N-end rule pathway is a conserved pathway for the control of protein turnover, through which the clipping or modification of amino acids from the amino-terminus of a protein leads to an interaction with PROTEOLYSIS 6 (PRT6; an N-recognin E3 ligase) and 26S proteasome-mediated proteolysis. Previously,…

Evidence for mid-Holocene rice domestication in the Americas
Blog, Plant Science Research Weekly, Research, Research BlogRice is one of our most important crops, and previous work has indicated that it was domesticated independently in Asia (Oryza sativa) and Africa (Oryza glaberrima). Using archeological approaches, Hilbert et al. for the first time show evidence for domestication of a rice species (Oryza sp.) in the…

Expression and purification of unstable proteins using an ASK-assisted system
Blog, Plant Science Research Weekly, Research, Research BlogUbiquitin-mediated protein degradation is important in many plant processes (growth, development, responses to stress) but little is known about F-box proteins, a key component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, due to their unstable protein structures. Li et al. investigated…

Small peptides, big importance: Small, secreted peptides as novel regulators of symbiosis and nutrient acquisition
Blog, Plant Science Research Weekly, Research, Research BlogIt is becoming increasingly evident that small, secreted peptides (SSPs) are important regulators of plant development and responses to stress. Traditional gene prediction algorithms are biased toward larger coding sequences and have therefore been inadequate in the hunt for plant SSPs. To address this…

Improving crops by genome editing
Blog, Plant Science Research Weekly, Research, Research BlogIntroducing new desirable traits in domesticated crops takes time and is limited by the need for existing trait variation in members of the same species or closely related species. Rodríguez-Leal et al. propose a system by which variation in a quantitative trait can be generated by editing the promoter…

The genome of Quenopodium quinoa, a halophytic pseudocereal
Blog, Plant Science Research Weekly, Research, Research BlogQuenopodiium quinoa is a highly nutritive and facultative halophyte pseudocereal whose cultivation has increased 10 fold in the last decades. However, the adaption to non-native areas is not easy to achieve and the limited genetic resources do not allow a breeding program. Zou and collaborators have…
