Recent Posts

Nanoscale movements of cellulose microfibrils in primary cell walls ($)

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Cell walls are complex mixtures of cellulose microfibrils, proteins and other materials. Their mechanical properties can be measured and modeled, but it is not always simple to translate these measurements to changes at the molecular level. Zhang et al. used atomic force microscopy to provide an unprecedented…

Opposing, polarity-driven nuclear migrations underpin asymmetric divisions in stomatal patterning (Curr. Biol.)

Asymmetric cell divisions (ACDs) are often used by organisms to generate different cell sizes, each adopting a different cell fate. In plants, stomatal development provides an example of an ACD-derived patterning mechanism. Several proteins are known to be implicated in ACDs, like BREAKING OF ASYMMETRY…

Follow that Protein: SNAP-tagging Permits High-resolution Protein Localization

Protein analysis relies heavily on the production of ‘tagged’ proteins, i.e. recombinant proteins that contain both a functional peptide sequence and a peptide or chemical label (the ‘tag’). Common tags include fluorescent proteins (FPs), which make tagged proteins easier to detect, as well as…

Roles for this ROP GTPase in subcellular and tissue-level patterning (Plant Cell)

Rho-of-plants (ROP) proteins are master regulators of cell polarity within plants and influence cell wall deposition, tissue development, and several signaling processes. However, they are quite difficult to study: attempts to form a fluorescent ROP fusion protein have proven elusive, and there are multiple…

Chemical labeling of proteins in live plant cells

Iwatate et al. demonstrate that SNAP-tag technology can be used to label plant cell proteins. Plant Cell https://doi.org/10.1105/tpc.20.00439 By Ryu J. Iwatatea,b, Akira Yoshinaria, Noriyoshi Yagia, Wolf B. Frommera,c, and Masayoshi Nakamuraa a Institute of Transformative Bio-Molecules (WPI-ITbM),…